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• Book

  Structural genomics and drug discovery : methods and protocols

  edited by Wayne F. Anderson.

  Contents:
  Data Management in the Modern Structural Biology and Biomedical Research Environment
  Structural Genomics of Human Proteins
  Target Selection for Structural Genomics of Infectious Diseases
  Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery
  High Throughput Cloning for Biophysical Applications
  Expression and Solubility Testing in a High Throughput Environment
  Protein Production for Structural Genomics Using E. coli Expression
  Eukaryotic Expression Systems for Structural Studies
  Automated Cell-free Protein Production Methods for Structural Studies
  Parallel Protein Purification
  Oxidative Refolding from Inclusion Bodies
  High throughput Crystallization Screening
  Screening Proteins for NMR Suitability
  Salvage or Recovery of Failed Targets by in situ Proteolysis
  Salvage of Failed Protein Targets by Reductive Alkylation
  Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy
  Data Collection for Crystallographic Structure Determination
  Structure, Determination, Refinement, and Validation
  Virtual High-Throughput Ligand Screening
  Ligand Screening using Fluorescence Thermal Shift Analysis (FTS)
  Ligand Screening using Enzymatic Assays
  Ligand Screening using NMR
  Screening Ligands by X-ray Crystallography
  Case Study Structural Genomics and Human Protein Kinases.

  Digital Access Springer 2014
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• Article

  Aneurismectomia e by-pass aorto-coronarico. Risultati immediati ed a distanza in 56 pazienti. [Aneurysmectomy and aortocoronary bypass. Immediate and long-term results of 56 patients].

  Corbara F, Gallucci V, Casarotto D, Chioin R, Palù M, Roman F, Stritoni P, Fasoli G.

  G Ital Cardiol. 1979;9(8):836-44.

  56 pts. who underwent left ventricular aneurismectomy were studied. Clinical improvement and lat post-operatory mortality rate have been evaluated. 39 pts. (II group) also had aortocoronary bypass and other surgical procedures performed at the time of the aneurismectomy. Group I (no other surgery beside the aneurismectomy) and group II did not significant differences in the pre-operatory period and were, therefore, comparable. The total operatory mortality has been of 14% (17.6% in group I and 12.8% in group II). By using myocardial protection the mortality dropped to 5.8%. A significant difference between deceased and survived pts. was noted in the following parameters: cardiac index, A-V oxygen difference, extracorporeal circulation time and the number of diseased coronary arteries (P < 0.001-0.005). Only 2 pts., both in group II, had a late death. After the operation 32 pts. became asymptomatic. Five pts. remained symptomatic: 3 continued to complain of angina and 2 to show signs of left ventricular failure; ventricular arrhythmias were still present in 6 pts. post-operatively (compared to 16 pts. pre-op.). The data suggested that aneurismectomy, associated with aorto-coronary bypass and myocardial protection, has an acceptable operatory risk, particulary in pts. with a good residual ventricular function. Except for ventricular arrhythmias clinical results are very good and late mortality rate is low

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