Bookedited by Wayne F. Anderson.
Contents:
Data Management in the Modern Structural Biology and Biomedical Research Environment
Structural Genomics of Human Proteins
Target Selection for Structural Genomics of Infectious Diseases
Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery
High Throughput Cloning for Biophysical Applications
Expression and Solubility Testing in a High Throughput Environment
Protein Production for Structural Genomics Using E. coli Expression
Eukaryotic Expression Systems for Structural Studies
Automated Cell-free Protein Production Methods for Structural Studies
Parallel Protein Purification
Oxidative Refolding from Inclusion Bodies
High throughput Crystallization Screening
Screening Proteins for NMR Suitability
Salvage or Recovery of Failed Targets by in situ Proteolysis
Salvage of Failed Protein Targets by Reductive Alkylation
Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy
Data Collection for Crystallographic Structure Determination
Structure, Determination, Refinement, and Validation
Virtual High-Throughput Ligand Screening
Ligand Screening using Fluorescence Thermal Shift Analysis (FTS)
Ligand Screening using Enzymatic Assays
Ligand Screening using NMR
Screening Ligands by X-ray Crystallography
Case Study Structural Genomics and Human Protein Kinases.