Bookedited by Vincent Ossipow, Nicolas Fischer.
Contents:
Antigen production for monoclonal antibody generation / Giovanni Magistrelli and Pauline Malinge
Method for the generation of antibodies specific for site and posttranslational modifications / Hidemasa Goto and Masaki Inagaki
Immunization, hybridoma generation, and selection for monoclonal antibody production / Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo
Hybridoma technology for the generation of rodent mAbs via classical fusion / Efthalia Chronopoulou [and three others]
Generation of rabbit monoclonal antibodies / Pi-Chen Yam and Katherine L. Knight
Screening hybridomas for cell surface antigens by high-throughput homogeneous assay and flow cytometry / Alejandro Uribe-Benninghoff [and four others]
Screening and subcloning of high producer transfectomas using semisolid media and automated colony picker / Suba Dharshanan and Cheah Swee Hung
Design and generation of synthetic antibody libraries for phage display / Gang Chen and Sachdev S. Sidhu
Selection and screening using antibody phage display libraries / Patrick Koenig and Germaine Fuh
Yeast surface display for antibody isolation : library construction, library screening, and affinity maturation / James A.Van Deventer and Karl Dane Wittrup
Human B cell immortalization for monoclonal antibody production / Joyce Hui-Yuen, Siva Koganti, and Sumita Bhaduri-McIntosh
Using next-generation sequencing for discovery of high- frequency monoclonal antibodies in the variable gene repertoires from immunized mice / Ulrike Haessler and Sai T. Reddy
Cloning, reformatting, and small-scale expression of monoclonal antibody isolated from mouse, rat, or hamster hybridoma / Jeremy Loyau and François Rousseau
Cloning of recombinant monoclonal antibodies from hybridomas in a single mammalian expression plasmid / Nicole Müller-Sienerth [and three others]
Monoclonal antibody purification by ceramic hydroxyapatite chromatography / Larry J. Cummings, Russell G. Frost, and Mark A. Snyder
Rapid purification of monoclonal antibodies using magnetic microspheres / Pauline Malinge and Giovanni Magistrelli
Generation of cell lines for monoclonal antibody production / Krista Alvin and Jianxin Ye
Expression and purification of recombinant antibody formats and antibody fusion proteins / Martin Siegemund [and four others]
Purification of antibodies and antibody fragments using CaptureSelect [trademark] affinity resins / Pim Hermans, Hendrik Adams, and Frank Detmers
Reformatting of scFv antibodies into the scFv-Fc format and their downstream purification / Emil Bujak [and three others]
Antibody V and C domain sequence, structure, and interaction analysis with special reference to IMGT® / Eltaf Alamyar [and three others]
Measuring antibody affinities as well as the active concentration of antigens present on a cell surface / Palaniswami Rathanaswami
Determination of antibody structures / Robyn L. Stanfield
Affinity maturation of monoclonal antibodies by multi-site- directed mutagenesis / Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo
Epitope mapping with membrane-bound synthetic overlapping peptides / Terumi Midoro-Horiuti and Randall M. Goldblum
Epitope mapping by epitope excision, hydrogen/deuterium exchange, and peptide-panning techniques combined with in silico analysis/ Nicola Clementi [and five others]
Fine epitope mapping based on phage display and extensive mutagenesis of the target antigen / Gertrudis Rojas
Epitope mapping with random phage display library / Terumi Midoro-Horiuti and Randall M. Goldblum
Epitope mapping of monoclonal and polyclonal antibodies using bacterial cell surface display / Anna-Luisa Volk, Francis Jingxin Hu, and Johan Rockberg
Ion exchange-high-performance liquid chromatography (IEX-HPLC) / Marie Corbier, Delphine Schrag, and Sylvain Raimondi
Size exclusion-high-performance liquid chromatography (SEC-HPLC) / Delphine Schrag, Marie Corbier, and Sylvain Raimondi
N-glycosylation characterization by liquid chromatography with mass spectrometry / Song Klapoetke
Fc engineering of antibodies and antibody derivatives by primary sequence alteration and their functional characterization / Stefanie Derer [and six others]
Labeling and use of monoclonal antibodies in immunofluorescence : protocols for cytoskeletal and nuclear antigens / Christoph R. Bauer
Generation and use of antibody fragments for structural studies of proteins refractory to crystallization / Stephen J. Stahl, Norman R. Watts, and Paul T. Wingfield
Antibody array generation and use / Carl A.K. Borrebaeck and Christer Wingren.