Bookedited by Paul Birch, John T. Jones, Jorunn I.B. Bos.
Contents:
Galaxy as a Platform for Identifying Candidate Pathogen Effectors
Bioinformatic analysis of expression data to identify effector candidates
Two-dimensional data binning for the analysis of genome architecture in filamentous plant pathogens and other eukaryotes
On The Statistics Of Identifying Candidate Pathogen Effectors
High-throughput imaging of plant immune responses
In vivo protein-protein interaction studies with BiFC: conditions, cautions and caveats
Particle bombardment-mediated transient expression to identify localization signals in plant disease resistance proteins and target sites for the proteolytic activity of pathogen effectors
Purification of fungal haustoria from infected plant tissue by flow cytometry
Functional Characterisation of Nematode Effectors in Plants
Silencing of aphid genes by feeding on stable transgenic Arabidopsis thaliana
Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids
A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana
Simple quantification of in planta fungal biomass
Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum
DIGE-ABPP by click chemistry: Pairwise comparison of serine hydrolase activities from the apoplast of infected plants
A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector Host Protein Complexes
An Arabidopsis and Tomato Mesophyll Protoplast System for fast Identification of early MAMP-triggered Immunity-Suppressing Effectors
Production of RXLR effector proteins for structural analysis by X-ray crystallography
The Dos and Donts of effectoromics
Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice
A Bacterial Type III Secretion Based Delivery System for Functional Assays of Fungal Effectors in Cereals
Capture arrays to annotate resistance genes in plant genomes and to accelerate plant resistance gene discovery.