Bookedited by Klemens J. Hertel.
Contents:
The pre-mRNA splicing reaction / Somsakul Pop Wongpalee and Shalini Sharma
Diversity and evolution of spliceosomal systems / Scott William Roy and Manuel Irimia
Mechanisms of spliceosomal assembly / Ni-ting Chiou and Kristen W. Lynch
Alternative pre-mRNA splicing / Stacey D. Wagner and J. Andrew Berglund
Regulation of alternative pre-mRNA splicing / Miguel B. Coelho and Christopher W.J. Smith
Introduction to cotranscriptional RNA splicing / Evan C. Merkhofer, Peter Hu, and Tracy L. Johnson
Chromatin and splicing / Nazmul Haque and Shalini Oberdoerffer
Preparation of splicing competent nuclear extracts / Chiu-Ho T. Webb and Klemens J. Hertel
Preparation of yeast whole cell splicing extract / Elizabeth A. Dunn and Stephen D. Rader
Efficient splinted ligation of synthetic RNA using RNA ligase / Martha R. Stark and Stephen D. Rader
In vitro assay of pre-mRNA splicing in mammalian nuclear extract / Maliheh Movassat, William F. Mueller, and Klemens J. Hertel
Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract / William F. Mueller and Klemens J. Hertel
In vitro systems for coupling RNAP II transcription to splicing and polyadenylation / Eric G. Folco and Robin Reed
Isolation and accumulation of spliceosomal assembly intermediates / Janine O. Ilagan and Melissa S. Jurica
Complementation of U4 snRNA in S. cerevisiae splicing extracts for biochemical studies of snRNP assembly and function / Martha R. Stark and Stephen D. Rader
Expression and purification of splicing proteins from mammalian cells / Eric Allemand and Michelle L. Hastings
Single molecule approaches for studying spliceosome assembly and catalysis / Eric G. Anderson and Aaron A. Hoskins
Cell-based splicing of minigenes / Sarah A. Smith and Kristen W. Lynch
Quantifying the ratio of spliceosome components assembled on pre-mRNA / Noa Neufeld, Yehuda Brody, and Yaron Shav-Tal
Antisense methods to modulate pre-mRNA splicing / Joonbae Seo, Eric W. Ottesen, and Ravindra N. Singh
Using yeast genetics to study splicing mechanisms / Munshi Azad Hossain and Tracy L. Johnson
Medium throughput analysis of alternative splicing by fluorescently labeled RT-PCR / Ryan Percifield, Daniel Murphy, and Peter Stoilov
Chromatin immunoprecipitation approaches to determine co-transcriptional nature of splicing / Nicole I. Bieberstein, Korinna Straube, and Karla M. Neugebauer
Computational approaches to mine publicly available databases / Rodger B. Voelker, William A. Cresko, and J. Andrew Berglund
Approaches to link RNA secondary structures with splicing regulation / Mireya Plass and Eduardo Eyras
Methods to study splicing from high-throughput RNA sequencing data / Gael P. Alamancos, Eneritz Agirre, and Eduardo Eyras
Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq / Chengguo Yao, Lingjie Weng, and Yongsheng Shi
Predicting alternative splicing / Yoseph Barash and Jorge Vaquero Garcia.