Bookedited by Mikiko C. Siomi.
Contents:
Chromatin Immunoprecipitation Assay of Piwi in Drosophila / Hang Yin and Haifan Lin
Drosophila Germline Stem Cells for In Vitro Analyses of PIWI-Mediated RNAi / Yuzo Niki, Takuya Sato, Takafumi Yamaguchi, Ayaka Saisho, Hiroshi Uetake, and Hidenori Watanabe
RNAi and Overexpression of Genes in Ovarian Somatic Cells / Kuniaki Saito
Making piRNAs In Vitro / Shinpei Kawaoka, Susumu Katsuma, and Yukihide Tomari
A Framework for piRNA Cluster Manipulation / Ivan Olovnikov, Adrien Le Thomas, and Alexei A. Aravin
Biochemical and Mass Spectrometric Analysis of 3ʹ-End Methylation of piRNAs / Takeo Suzuki, Kenjyo Miyauchi, Yuriko Sakaguchi, and Tsutomu Suzuki
HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins / Anastassios Vourekas and Zissimos Mourelatos
DNA Methylation in Mouse Testes / Satomi Kuramochi-Miyagawa, Kanako Kita-Kojima, Yusuke Shiromoto, Daisuke Ito, Hirotaka Koshima, and Toru Nakano
Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes / Michael Reuter and Ramesh S. Pillai
Small RNA Library Construction from Minute Biological Samples / Jessica A. Matts, Yuliya Sytnikova, Gung-wei Chirn, Gabor L. Igloi, and Nelson C. Lau
Analysis of sDMA Modifications of PIWI Proteins / Shozo Honda, Yoriko Kirino, and Yohei Kirino
Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries / Sergey Shpiz and Alla Kalmykova
Combined RNA/DNA Fluorescence In Situ Hybridization on Whole-Mount Drosophila Ovaries / Shpiz, Sergey Lavrov, and Alla Kalmykova
Fast and Accurate Method to Purify Small Noncoding RNAs from Drosophila Ovaries / Thomas Grentzinger and Séverine Chambeyron
Isolation of Zebrafish Gonads for RNA Isolation / Hsin-Yi Huang and René F. Ketting
Small RNA Library Construction for High-Throughput Sequencing / Jon McGinn and Benjamin Czech
Analysis of Piwi-Loaded Small RNAs in Terahymena / Tomoko Noto, Henriette M. Kurth, and Kazufumi Mochizuki
Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs / Hailong Wang, Haidong Huang, and Dahua Chen
Isolation and Bioinformatic Analyses of Small RNAs Interacting with Germ Cell-Specific Argonaute in Rice / Reina Komiya and Ken-Ichi Nonomura.