Bookedited by Elisa Vicenzi and Guido Poli.
Contents:
Virion attachment and entry : HIV gp120 env biotinylation, gp120 env, or integrin ligand-binding assay / Claudia Cicala and James Arthos
CryoEM analysis of capsid assembly and structural changes upon interactions with a host restriction factor, TRIM5[alpha] / Gongpu Zhao and Peijun Zhang
The fate of HIV-1 capsid : a biochemical assay for HIV-1 uncoating / Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero
The cyclosporin A washout assay to detect HIV-1 uncoating in infected cells / Amy E. Hulme and Thomas J. Hope
Imaging HIV-1 nuclear pre-integration complexes / Anna Cereseto and Mauro Giacca
HIV-1 reverse transcription / Andrea Cimarelli and Jean-Luc Darlix
RNase H : specificity mechanisms of action, and antiviral target / Karin Moelling, Felix Broecker, and John E. Kerrigan
HIV-1 chromatin, transcription, and the regulatory protein Tat / Laurence Colin, Eric Verdin, and Carine Van Lint
HIV-1 Rev function and RNA nuclear-cytoplasmic export / Alan Cochrane
HIV-1 accessory proteins : Nef / Anke Heigele [and three others]
HIV-1 accessory proteins : VpR / Richard Y. Zho and Michael I. Bukrinsky
HIV-1 accessory proteins : Vpu and Vif / Amy Andrew and Klaus Strebel
SIVSM/HIV-2 Vpx proteins : function and uses in the infection of primary myeloid cells / Gregory Berger and Andrea Cimarelli
Imaging of HIV assembly and release / Barbara Müller and Jacomine Krijnse-Locker
HIV-1 isolation from infected peripheral blood mononuclear cells / Stefania Dispinseri [and four others]
Determination of HIV-1 co-receptor usage / Mariangela Cavarelli and Gabriella Scarlatti
The macrophage and HIV : basic concepts and methodologies / Suzanne Gartner
HIV infection of dendritic cells / Najla Nasr [and three others]
Histocultures (tissue explants) in human retrovirology / Anush Arakelyan [and four others]
Single-copy quantification of HIV-1 in clinical samples / Ann Wiegand and Frank Maldarelli
Quantification of total HIV1-DNA in peripheral blood mononuclear cells / Christine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël
HIV-1 based lentiviral vectors / Ying Poi Liu and Ben Berkhout
Quantification of miRNA by poly(A)-RT-qPCR arrays and verification of target sites in HIV-1 using a one-LTR infectious molecular clone / Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang
Investigating human T cell lymphotropic retrovirus (HTLV) Tax function with molecular and immunophenotypic techniques / Greta Forlani, Roberto S. Accolla, and Giovanna Tosi
Proviral load determination of HTLV-1 and HTLV-2 in patients' peripheral blood mononuclear cells by real-time PCR / Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni
Quantitative analysis of human T-lymphotropic virus type 1 (HTLV-1) gene expression using nucleo-cytoplasmic fractionation and splice junction-specific real-time RT-PCR (qRT-PCR) / Ilaria Cavallari, Francesca Rende, and Vincenzo Ciminale.