Bookedited by Peter E. Nielsen, Daniel H. Appella.
Summary: Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Peptide Nucleic Acids: Methods and Protocols, Second Editionserves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.
Contents:
MiniPEG-PNA / Arunava Manna [and others]
Cyclopentane peptide nucleic acids / Ethan A. Englund, Ning Zhang, and Daniel H. Appella
Chiral PNAs with constrained open-chain backbones / Roberto Corradini [and others]
Synthesis, characterization, and evaluation of radiometal-containing peptide nucleic acids / Holger Stephan, Christian Foerster, and Gilles Gasser
Preparation of metal-containing peptide nucleic acid bioconjugates on the solid phase / Gilles Gasser
Formation and characterization of PNA-containing heteroquadruplexes / Bruce A. Armitage
Sequence selective recognition of double-stranded RNA using triple helix-forming peptide nucleic acids / Thomas Zengeya, Pankaj Gupta, and Eriks Rozners
Assembly of PNA-tagged small molecules, peptides, and carbohydrates onto DNA templates : programming the combinatorial pairing and inter-ligand distance / Nicolas Winssinger [and others]
Site-selective scission of human genome using PNA-based artificial restriction DNA cutter / Kenichiro Ito and Makoto Komiyama
PNA openers and their applications for bacterial DNA diagnostics / Irina V. Smolina and Maxim D. Frank-Kamenetskii
DNA-Templated Native chemical ligation of functionalized peptide nucleic acids : a versatile tool for single base-specific detection of nucleic acids / Alexander Roloff [and others]
Use of peptide nucleic acids (PNAs) for genotyping by solution and surface methods / Stefano Sforza [and others]
Analysis of PNA Hybridization by surface plasmon resonance / Bruce A. Armitage
PNA fluorescent in situ hybridization (FISH) for rapid microbiology and cytogenetic analysis / Henrik Stender, Brett Williams, and James Coull
Rapid miRNA imaging in cells using fluorogenic templated staudinger reaction between PNA-based probes / Katarzyna Gorska and Nicolas Winssinger
Cellular delivery of peptide nucleic acids (PNAs) / Takehiko Shiraishi and Peter E. Nielsen
Peptide nucleic acid-mediated recombination for targeted genomic repair and modification / Erica B. Schleifman and Peter M. Glazer
Antisense effects of PNAs in bacteria / Shan Goh, Jem Stach, and Liam Good.