Bookedited by Arun K. Shukla.
Summary: Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.
Contents:
Engineering Escherichia coli for functional expression of membrane proteins
ACEMBLing a multiprotein transmembrane complex: the functional SecYEG-SecDF-YajC-YidC holotranslocon protein secretase/insertase
Expression of purification of OsVDAC4
Membrane protein expression in Lactococcus lactis
An unconventional anaerobic membrane protein production system based on Wolinella succinogenes
Membrane protein expression and analysis in yeast
Heterologous expression of G-protein-coupled receptors in yeast
Recombinant G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization
Baculovirus-mediated expression of GPCRs in insect cells
Expression of membrane proteins in the eyes of transgenic Drosophila melangaster
Rapid method to express and purify human membrane protein using the Xenopus Oocyte system for functional and low-resolution structural anlaysis
Expression of G protein-coupled receptors in mammalian cells
Construction of stable mammalian cell lines for inducible expression of G protein-coupled receptors
Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI- cells using a PiggyBac inducible system
Semliki forest virus-based expression of recombinant GPCRs
Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs
Liposome reconstitution and transport assay for recombinant transporters
Lipid reconstitution and recording of recombinant ion channels
Reconstitution of membrane proteins: a GPCR as an example
Ion channel reporter for monitoring the activity of engineered GPCRs
Methods for labeling skeletal muscle ion channels site-specifically with fluorophores suitable for FRET-based structural analysis
Structural and fucntional studies of NirC from Salmonella typhimurium
Surface plasmon resonance analysis of seven-transmembrane receptors
Cross-linking strategies to study peptide ligand-receptor ineractions
From recombinant expression to crystals: a step-by-step guide to GPCR crystallography
Structure-based biophysical analysis of the interaction of rhodopsin with G protein and arrestin.