BookManfred Dietel, Christian Wittekind, Gianni Bussolati, Moritz von Winterfeld, editors.
Summary: This book presents an overview of the most important current developments in the pre-analytical handling of tissue. It addresses in particular potential ways to improve the situation whereby methods employed in the pre-analytical phase ? the period from surgical removal of tissue to the start of pathological processing ? have remained essentially unchanged for decades with only modest standardization. It is examined how the pre-analytical period can be optimized, resulting not only in an increase in diagnostic quality but also in a reduction in processing time and costs. Among the key topics examined are the so-called cold ischemia time between tissue removal and fixation, the potential superiority of vacuum-based preservation over immediate formalin fixation, two-temperature fixation, molecular analysis methods, and the pre-analytics of specimens from particular tissues. Readers will find this book to be an important update that reveals the full importance of the pre-analytical phase for quality of pathological work-up.
Contents:
What Is Pre-Analytics in Surgical Pathology?
Histologic Validation of Vacuum Sealed Formalin
Free Tissue Preservation and Transport System
Pre-Analytics and Molecular Pathology
Experiences in Vacuum Preparation of Routine Specimens
Tissue Heterogeneity as a Pre-Analytical Source of Variability
Vacuum Fixation of Breast Specimens Before Grossing
How Do Short Fixation and Rapid Microwave Processing Affect Her2 Testing?
Current Projects in Pre-Analytics ? Where to Go?
Nucleic Acid Extraction from FFPE Tissue as Critical Preanalytic Step for NGS Applications
Experiences With Pre-Analytics of Lung Specimen
Experiences With Pre-Analytics of GI-Specimen
Bone Marrow Work-Up
Two-Temperature Fixation Preserves Activation Status with High Efficiency
Towards the Lean Lab. The Industry Challenge. .