Bookedited by Donald H. Burke-Aguero.
Summary: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, and ligand interactions and thermodynamics.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in biomineralization scienceContains sections on such topics as riboswitch discovery and valid.
Contents:
Riboswitch discovery by combining RNA-seq and genome-wide indentification of transcriptional start sites
Discoverying human RNA aptamers by structure-based bioinformatics and genome-based in vitro selection
Affinity purification of in vitro transcribed RNA with homogenous ends using a 3'-ARiBo tag
Deoxyribozyme-mediated ligation for incorporating EPR spin labels and reporter groups into RNA
A flexible, scalable method for preparation of homogenous aminoacylated tRNAs
Synthesis of a biotinylated photocleavable nucleotide monophosphate for the preparation of natively folded RNAs
Chemo-enzymatic synthesis of selectively 13C/15N-labeled RNA for NMR structural and dynamics studies
SHAPE analysis of small RNAs and riboswitches
Experimental approaches for measuring pKa's in RNA and DNA
Crystallographic analysis of TPP riboswitch binding by small-molecule ligands discovered through fragment-based drug discovery approaches
Methods for using new conceptual tools and parameters to assess RNA structure by small-angle X-ray scattering
Use of 19F NMR methods to probe conformational heterogeneity and dynamics of exchange in functional RNA molecules
Site-directed spin-labeling strategies and electron paramagetic resonance spectroscopy for large riboswitches
Using sm-FRET and denaturants to reveal folding landscapes
Riboswitch structure and dynamics by smFRET microscopy
Ribosome structure and dynamics by smFRET microscopy
Unraveling the thermodynamics and kinetics of RNA assembly: surface plasmon resonance, isothermal titration calorimetry, and circular dichroism
ITC analysis of ligand binding to PreQ1 riboswitches
Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance
The AdoCbl-riboswitch interaction investitaged by in-line probing and surface plasmon resonance spectroscopoy (SPR)
Assessing RNA interactions with proteins by DRaCALA.