BookHuangui Xiong, Howard E. Gendelman, editors.
Summary: Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a blueprint for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.
Contents:
Part I. Neurohistological Techniques
Brain Tissue Preparation, Sectioning, and Staining / Jingdong Zhang and Huangui Xiong
Brain Stereotaxic Injection / Jingdong Zhang and Huangui Xiong
Neuronal Tract Tracing with Light and Electron Microscopy / Jingdong Zhang and Huangui Xiong
Stereological Analysis / Kristi M. Anderson, Adam M. Szlachetka and R. Lee Mosley
Part II. In Vitro Preparations
Preparation and Use of Rodent Hippocampal Slices / Huangui Xiong and Jianxun Xia
Single-Cell Neuronal Dissociation for Electrophysiological Studies / Yu-Long Li
Isolation and Culture of Human Neurons, Microglia, and Astrocytes / Li Wu and Santhi Gorantla
Isolation and Culture of Neural Stem/Progenitor Cells / Hui Peng, Qiang Chen and Jialin Zheng
Isolation of Mitochondria from Brain Tissue and Cells / Changhai Tian and Jialin Zheng
Isolation of Synaptosomes from Archived Brain Tissues / Gurudutt Pendyala, James L. Buescher and Howard S. Fox
Xenopus Oocytes Isolation and Microinjection / Huangui Xiong
Part III. Leukocyte Isolation and Application in Neuroscience
Centrifugal Elutriation for Studies of Neuroimmunity / Santhi Gorantla, Myhanh Che and Howard E. Gendelman
Flow Cytometry in Neuroscience Research / Charles Kuszynski
Part IV. Standard Laboratory Nucleic Acid and Protein Detections
Western Blotting Technique in Biomedical Research / Jianuo Liu, James Haorah and Huangui Xiong
Polymerase Chain Reaction (PCR) and Real-Time PCR / Georgette D. Kanmogne
Part V. Nanomedicine
Nanoformulations / Xin-Ming Liu and Tatiana K. Bronich
Neuronanomedicine / JoEllyn M. McMillan, Xin-Ming Liu and Howard E. Gendelman
Part VI. Bioimaging
Confocal Imaging of Nerve Cells / You Zhou
Magnetic Resonance Imaging / Yutong Liu
X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging / Katherine A. Estes, Jacob C. Peterson, Adam M. Szlachetka and R. Lee Mosley
Noninvasive Neurophysiological Imaging with Magnetoencephalography / Tony W. Wilson
Multiphoton Brain Imaging / Anna Dunaevsky
Part VII. Neuroelectrophysiology
Techniques for Extracellular Recordings / Jingdong Zhang, Jianxun Xia and Huangui Xiong
Blind Patch-Clamp Recordings from Rodent Hippocampal Slices / Jianxun Xia and Huangui Xiong
Whole-Cell Patch-Clamp Recording / Matthew J. Van Hook and Wallace B. Thoreson
Part VIII. Immunohistochemistry and Autoradiography
Analysis of Receptor Binding and Quantitative Autoradiography / L. Charles Murrin
Techniques in Immunohistochemistry and Immunocytochemistry / Yang Yuan and Jyothi Arikkath
Part IX. Analysis of Gene Expression
Protein Identification by Mass Spectrometry: Proteomics / Melinda Wojtkiewicz, Kelley Barnett and Pawel Ciborowski
DNA Microarrays as a Tool for Neurosciences Research / James D. Eudy, Lynette Smith
Metabolomics / Yazen Alnouti
Bioinformatic Methods and Resources for Neuroscience Research / Chittibabu Guda
Part X. Animal Models: Behavior and Pathology
Motor Function in Rodent Models of Neurodegenerative Disorders / Jessica A. Hutter Saunders, Max V. Kuenstling, Robert A. Weir, R. Lee Mosley and Howard E. Gendelman
Humanized Mice / Larisa Y. Poluektova and Edward Makarov
Animal Models for PD and ALS / Max V. Kuenstling, Adam M. Szlachetka and R. Lee Mosley
Animal Models of Alzheimer's Disease / Tomomi Kiyota
Animal Models: Behavior and Pathology: Preclinical Assessment of the Putative Cognitive Deficits in HAND / Landhing M. Moran, Rosemarie M. Booze and Charles F. Mactutus.