BookShirley Pease, Thomas L. Saunders, editors ; ISTT, International Society for Transgenic Technologies.
Contents:
Patent and licensing issues in transgenic technology / Karen S. Canady
Global resources : including gene trapped ES cell clones
is your gene already knocked out / Lluis Montoliu
Designing transgenes for optimal expression / Eduardo Molto, Cristina Vicente-Garcıa, and Lluis Montoliu
Gene targeting vector design for embryonic stem cell modifications / Thomas L. Saunders
Transgenic production benchmarks / Thomas J. Fielder and Lluis Montoliu
Generation of transgenic mice by pronuclear microinjection / Katja Becker and Boris Jerchow
Generation of transgenic rats using microinjection of plasmid DNA or lentiviral vectors / Severine Menoret ... [et al.]
Generation of transgenic animals by use of YACs / Almudena Fernandez, Diego Munoz, and Lluis Montoliu
BAC transgenes, DNA purification, and transgenic mouse production / Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders
Generation of transgenic animals with lentiviral vectors / Carlos Lois
Vertebrate transgenesis by transposition / Aron Geurts, Darius Balciunas, and Lajos Mates
Rat spermatogonial stem cell-mediated gene transfer / Karen M. Chapman ... [et al.]
Mouse cloning by nuclear transfer / Sayaka Wakayama, Nguyen Van Thuan, and Teruhiko Wakayama
Gene targeting in embryonic stem cells / Elizabeth D. Hughes and Thomas L. Saunders
Importance of mouse ES cell line selection / Wojtek Auerbach and Anna B. Auerbach
Tetraploid complementation assay / Marina Gertsenstein
Combining ES cells with embryos / Elizabeth Williams ... [et al.]
Derivation of murine ES cell lines / Kristina Nagy and Jennifer Nichols
Rat embryonic stem cell derivation and propagation / Ping Li ... [et al.]
Induced pluripotency : generation of iPS cells from mouse embryonic fibroblasts / Han Li ... [et al.]
Preparation and analysis of DNA for use in transgenic technology / Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark
Colony management / Karen Brennan
Cryopreservation / B. Pintado and J. Hourcade
Shipment of mice and embryos / Shirley Pease
Pathogen-free mouse rederivation by IVF, natural mating and hysterectomy / J.M. Sztein, R.J. Kastenmayer, and K.A. Perdue
Refinement, reduction, and replacement / Jan Parker-Thornburg.