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- JournalSummary: Videourology, the online peer-reviewed complement to Journal of Endourology, offers high-caliber video demonstrations of the latest cutting-edge robotic and laparoscopic surgical techniques and technologies as well as urologic endoscopic treatments designed to enable physicians to view and evaluate new minimally invasive and noninvasive techniques and apply them to their practice.Digital Access
- JournalSummary: Videourology, the online peer-reviewed complement to Journal of Endourology, offers high-caliber video demonstrations of the latest cutting-edge robotic and laparoscopic surgical techniques and technologies as well as urologic endoscopic treatments designed to enable physicians to view and evaluate new minimally invasive and noninvasive techniques and apply them to their practice.Digital Access
- ArticleEbener U, Welte K, Chandra P.Cancer Lett. 1979 Aug;7(4):179-88.A RNA-dependent DNA polymerase (RTase) was purified from human osteosarcoma tissue by successive column chromatography of the microsomal fraction on DEAE-cellulose (DE-23 and DE-52) and phosphocellulose. The purified enzyme has a molecular weight of about 68,000, a pH optimum of 8.1, a Mg2+ optimum of 0.8 mM, Mn2+ optimum of 1.0 mM and a KCl optimum of 60 mM. The enzyme transcribes (rA)n . (dT)12, (rC)n . (dG)12-18 and (2-O-methyl C)n . (dG)18, but is unable to transcribe (dA)n . (dT)10. The enzyme has no catalytic activity in the presence of oligodeoxynucleotide initiators alone, indicating the absence of terminal deoxynucleotidyl transferase. The purified enzyme is able to transcribe the heteropolymeric regions of a 70S RNA from R(Mu)LV. The presented data support the presence of a RNA-dependent DNA polymerase in human osteosarcoma tissue with biochemical properties, resembling those of C-type RNA tumor viruses.