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- JournalDigital AccessProviderVersionSpringerCINAHLv. 113-, 2000- Full text delayed 12 monthsAcad Search Premv. 119, no. 4-, Apr. 2003- Full text delayed 12 months
- ArticleBlok MC, van Dam K.Biochim Biophys Acta. 1979 Feb 02;550(3):527-42.Bacteriorhodopsin vesicles were associated with cellulose-nitrate filters impregnated with a solution of phospholipids in hexadecane. The generation of (photo)potentials upon illumination of the filter was studied in the absence and presence of ionophores, phospholipase A2, EDTA or polyene antibiotics. From these experiments the following conclusions are drawn. 1. Upon illumination of the filter, bacteriorhodopsin pumps protons into aqueous compartments located in the filter. 2. These aqueous compartments possibly do not originate from the compartments enclosed by the bacteriorhodopsin vesicles. Evidence is obtained that aqueous compartments are present in the surface layers of the lipid-impregnated filters. 3. The results are explained most easily by a mechanism, whereby fusion occurs between the vesicles and the lipids of the filter.