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  • Book
    Dracopoli, Nicholas C.
    Digital Access Wiley v. 00-, 1994-
    Print Access Request
    Location
    Version
    Call Number
    Items
    Books: General Collection (Downstairs)
    1994-2010.
    Loose-leaf for updating in supplement form.
    QH440.5 .C87
    4
  • Article
    McClain LD, Tomana M, Acton RT.
    Brain Res. 1978 Dec 22;159(1):161-71.
    Subcellular fractionation of C57BI/6J mouse brains produced a crude synaptosome preparation which contained virtually all of the Thy-1.2 antigenic activity of the isotonic whole brain homogenate. The Thy-1.2 was solubilized from the synaptosomes, following delipidation with acetone, by deoxycholate extraction. A glycoprotein fraction rich in Thy-1.2 was isolated from the bulk of the detergent-soluble material by lectin affinity chromatography. Fractionation of the lectin retentate by gel filtration chromatography produced a single peak of Thy-1.2 activity purified more than 2000-fold over the original homogenate. SDS polyacrylamide gel electrophoresis of this material revealed a single band which corresponded to an apparent molecular weight of 24,000. Amino acid composition data indicated that the protein portion of the molecule is similar to Thy-1.1 from mouse lymphoblastoid cells. Carbohydrate analysis revealed a qualitative similarity between mouse brain Thy-1.2 and Thy-1.1 from rat brain. Structural differences which could account for the Thy-1.1 and Thy-1.2 antigenic distinctions are apparently too subtle to be detected by compositional analysis.
    Digital Access Access Options
  • Book
    by F. C. Calkins and W. H. Emmons.
    Digital Access 1915