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  • Article
    Joseph DR, Kenney FT, Farrelly JG.
    Cancer Biochem Biophys. 1976 Aug;1(5):257-64.
    Polyguanylate- and poly(2'-O-methyl)uridylate-Sepharose have been prepared for affinity chromatography of DNA polymerases of viral origin (reverse transcriptase). Both cellular DNA polymerases and reverse transcriptase bind to polyguanylate-Sepharose. The cellular polymerases can be eluted from the column between 0.32 and 0.42 M NaCl while reverse transcriptase eluted between 0.56 and 0.78 M NaCl. However, only reverse transcriptase adheres to poly(2'-O-methyl)uridylate-Sepharose and can be eluted at approximately 0.35 M NaCl. The columns were used to partially purify RNA-dependent DNA polymerase from spleens of mice infected with Rauscher leukemia virus. The enzyme preparation is about 1300-fold purified and is inhibited by antiserum prepared against purified reverse transcriptase from Rauscher leukemia virus to the same extent as the virion enzyme.
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  • Book
    Thompson, E. P.
    Contents:
    Introduction: At the point of decay, by E. P. Thompson. - The climate of apathy: "Bastard" capitalism, by R. Samuel. The supply of demand, by S. Hall. - Between two worlds: Imperial retreat, by P. Worsley. Outside the whale, by E. P. Thompson. Breaking the chains of reason, by A. MacIntyre. - Out of apathy. Power at the base, by K. Alexander. Revolution, by E. P. Thompson.
    Print [1960]