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- ArticleTuboly S, Szent-Iványi T.Acta Microbiol Acad Sci Hung. 1976;23(1):23-8.Purified protein derivative from Mycobacterium bovis was separated into 6 fractions by electrophoresis at 1500 V/40 mA in pH 4.2 acetic acid-pyridine buffer. Further purification of one of the fractions on Sephadex G 25 column and by acid hydrolysis yielded antigen "PS" eliciting tuberculin reaction only in animals vaccinated with M. bovis BCG but not in those sensitized with M. avium and some fast-growing mycobacteria. The specificity of antigen "PS" was confirmed in vitro: the antigen induced blastic transformation only in lymphocytes of guinea pigs sensitized with M. bovis BCG.
- BookPublished under direction of Department of industrial relations, California, member of Western safety conference. As a report on official project no. 65-1-08-100 conducted under auspices of Work projects administration.Print 1940