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  Advances in Biomedical Engineering

  edited by David O. Cooney.

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  Books: General Collection (Downstairs)

  c1980

  R856 .A53 1980

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• Article

  Izmenenie khimicheskogo sostava i ustoĭchivosti k denaturatsii khromatina iz selezenki mysheĭ v protsesse immunogeneza [Changes in the chemical composition and physico-chemical characteristics of chromatin from spleens of mice during immunogenesis].

  Staĭkova MA, Stanchev BD, Nikolov TK.

  Mol Biol (Mosk). 1975 Mar-Apr;9(2):240-5.

  Comparative studies have been carried out for the chemical composition and physico-chemical characteristics of chromatin isolated from spleens of non-immunized and immunized mice. It is found that the chromatin from spleens of immunized mice contains significantly more non-histone proteins and RNA, while the quantity of histone proteins is unaltered. The melting temperatures of chromatin from spleens of non-immunized and immunized mice in 2.5-10(-4) M EDTA (pH 8.0) are 76.8+/-1.50 degrees and 74.4+/-1.10 degrees, respectively. DNA isolated from chromatin melts at 40 degrees. The melting of chromatin was followed in 5 mM sodium-cacodylate buffer (pH 7.0)+1.5-10(-4) M EDTA containing increasing concentrations of urea. The results show that during immunogenesis the changes of the chemical composition of the chromatin are accompanied by certain destabilisation of DNP complex.

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