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- ArticleWilloughby EM, Turner MJ, Sanderson AR.Eur J Immunol. 1978 Sep;8(9):628-34.The purification of detergent-solubilized rat histocompatibility antigens is described. The antigen may readily be incorporated into synthetic vesicles (liposomes) which appear to be unilamellar and between 0.1 microbeter and 0.2 micrometer in diameter. The addition of specific antibody leads to the agglutination and precipitation of the liposomes. An enzyme, horseradish peroxidase, may be incorporated into the trapped aqueous phase of the liposome, and its release by antibody and complement can be detected.