Search
Filter Results
- Resource Type
- Article1
- Journal1
- Journal Digital1
- Result From
- Lane Catalog1
- PubMed1
-
Year
- Journal Title
- Nucleic Acids Res1
Search Results
Sort by
- ArticleWinter G, Brownlee GG.Nucleic Acids Res. 1978 Sep;5(9):3129-39.A new general method of labelling the 2',3'-diol end of RNA with 32P has been devised suitable for gel sequencing. Poly(A) polymerase (E. coli) is incubated with the RNA and limiting amounts of alpha-32P-ATP. The mono-addition product is then cleaved with periodate and beta-eliminated with aniline, leaving the RNA terminally labelled with 3' 32P-phosphate. When applied to a model compound, tRNAPhe from E. coli, over 28 residues could be read from the 3' end.