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  • Journal
    Digital Access Da mar. 1884 a luglio 1887-
  • Book
    Chihua Fang, Wan Yee Lau, editors.
    Summary: This book presents the latest application of digital medical imaging technology in biliary tract surgery, including three-dimensional visualization preoperative evaluation, preoperative surgical planning, and simulated biliary surgery. Digital surgical diagnosis and treatment of cholecystolithisasis, bile duct stones, hepatolithiasis, gallbladder cancer, and bile duct cancer is described in details with more than 900 illustrations. Written by experts with wealthy of clinical experience, it will be a useful reference for general surgeons, as well as practitioners in related disciplines. Chihua Fang is a Professor and Director of Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China. He is the Chairman of Digital Medicine Branch of Chinese Medical Association. Wan Yee Lau is a Professor of Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. He is also the Academician of Chinese Academy of Sciences.

    Contents:
    Applied Anatomy of the Biliary Tract
    Application of Multi-Slice Spiral CT and MRI in Biliary Tract Surgery
    Imaging of Common Biliary Tract Diseases
    3D Visualization of Abdominal CT Images
    3D Printing Technology and Its Application in the Hepatobiliary and Pancreatic Surgery
    Virtual Surgical Instruments and Simulation Surgery
    Application of Indocyanine Green Fluorescence Imaging in Biliary Tract Surgery
    Application of Endoscopic Technique in Biliary Tract Surgery
    Construction and Clinical Application of 3D Visualization Platform for the Blood Supplying of the Extrahepatic Bile Duct
    Digital Surgical Diagnosis and Treatment of Cholecystolithiasis
    Digital Surgical Diagnosis and Treatment of Extrahepatic Bile Duct Stones
    Digital Surgical Diagnosis and Treatment of Hepatolithiasis
    Digital Surgical Diagnosis and Treatment of Biliary Dilatation
    3D Visual Diagnosis and Treatment of Bile Duct Injury
    Digital Surgical Diagnosis and Treatment of Gallbaldder Cancer
    Digital Diagnosis and Surgical Treatment of Bile Duct Cancer.
    Digital Access Springer 2021
  • Article
    Libbus BL, Schuetz AW.
    Cell Tissue Kinet. 1978 Jul;11(4):377-91.
    Initiation and progression of meiosis was followed in dispersed rat testicular cells by flow cytofluorometry and cytology. The DNA content of dissociated testicular cells of rats 6--30 days old, killed at daily intervals, was analysed by flow cytofluorometry using propidium iodide as a DNA-specific and quantitative fluorochrome. Testicular cells of a 6-day-old rat showed one peak of fluorescence. A second peak, at twice the modal channel number, appeared in testicular cells of 9-day-old animals. The number of cells under this peak increased progressively with age. A third peak, at half the channel number of the original one, appeared at 20 days and accounted for an increasing proportion of cells in testes taken from older rats. Cytological examination of the testicular tissue used for flow cytofluorometric analysis showed that preleptotene spermatocytes first appeared at 8 days after birth. Spermatids were first observed cytologically at 20 days after birth. The close temporal appearance of the fluorescence peaks with that of spermatocytes and spermatids, and the close association of the frequency of diploid and tetraploid cells as derived by flow cytofluorometry and cytology, indicated that the fluorescence peaks correspond--in order of increasing fluorescence--to spermatids, spermatogonia and somatic cells, and to spermatocytes. This conclusion was re-examined by analysing the ploidy levels of testicular cells of hypophysectomized or estradiol-treated by flow cytofluorodmetry. There was a loss of the haploid and tetraploid peaks subsequent to hypophysectomy. Estradiol dipropionate-treated rats, given weekly injections starting at 7 days of age, showed no appearance of the haploid peak and the regression of the tetraploid peak after an initial and transitory appearance. These results indicate that changes in ploidy levels that accompany the progression of meiosis in the testis were reflected in the sequential appearance of three fluorescence peaks as detected by flow cytofluorometry. The close correlation between the frequency of cell types as obtained by cytology and flow cytofluorometry indicates that the latter is a sensitive method for studying selected aspects of spermatogenesis in dissociated testicular cells.
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