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  • Book
    editor-in-chief: Rostam D. Farhadieh ; editors: Neil W. Bulstrode, Babak J. Mehrara, Sabrina Cugno.
    Digital Access
    Provider
    Version
    ScienceDirect
    2022
    ClinicalKey
    2022
  • Article
    Allen JW, Shuler CF, Latt SA.
    Somatic Cell Genet. 1978 Jul;4(4):393-405.
    5-Bromodeoxyuridine (BrdU) tablets with different physical characteristics are useful in a wide variety of studies requiring detection of DNA replication in vivo. These tablets can effect a high substitution of BrdU in DNA, thereby permitting sister chromatid differentiation in chromosomes stained with 33258 Hoechst alone or in conjunction with Giemsa. Baseline and cyclophosphamide-induced in vivo sister chromatid exchange frequencies in mouse spleen, marrow, and thymus were measured and found to be significantly greater than those in spermatogonia. Sister chromatid exchange analysis was also extended to mouse liver and to Chinese hamster and Armenian hamster marrow cells. Sister chromatid differentiation was observed in Armenian hamster meiotic tissue, and evidence for interhomolog chromatid exchange obtained.
    Digital Access Access Options