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  The Lancet. Digital Health

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  ScienceDirect

  v. 1-, 2019-

  ClinicalKey

  v. 1, 2019-
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• Article

  Lambda phage promoter used to enhance expression of a plasmid-cloned gene.

  Hedgpeth J, Ballivet M, Eisen H.

  Mol Gen Genet. 1978 Jul 11;163(2):197-203.

  A 50-fold (or greater) increase in the production of phage 21 repressor was obtained by construction of a plasmid in which the 21cI (repressor) gene could be transcribed from lambdaPL. The enhancement due to increased 21cI gene copy number and transcription from lambdaPL were at least five-fold and ten-fold, respectively. The plasmid was constructed in vitro by recombination of EcoRI-generated DNA fragments. The use of the DNA fragment containing lambdaPL in obtaining expression of cloned genes is discussed.

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