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  Enzyme engineering and evolution : general methods. First edition

  edited by Dan S. Tawfik.

  Contents:
  In vivo site-directed recombination (SDR) : An efficient tool to reveal beneficial epistasis / Javier Viña-Gonzalez and Miguel Alcalde
  A beginner's guide to molecular dynamics simulations and the identification of cross-correlation networks for enzyme engineering / Haoran Yu and Paul A. Dalby
  Exploration of enzyme diversity : High-throughput techniques for protein production and microscale biochemical characterization / Michal Vasina, Pavel Vanacek, Jiri Damborsky, and Zbynek Prokop
  Emulsion-based directed evolution of enzymes and proteins in yeast / Elizabeth C. Gardner, Ella J. Watkins, Jimmy Gollihar, and Andrew D. Ellington
  Remodeling enzyme active sites by stepwise loop insertion / Md Anarul Hoque, Yong Zhang, Zhi Li, Li Cui, and Yan Feng
  Consensus Finder web tool to predict stabilizing substitutions in proteins / Bryan J. Jones, Chi Nok Enoch Kan, Christine Luo, and Romas J. Kazlauskas
  The use of consensus sequence information to engineer stability and activity in proteins / Matt Sternke, Katherine W. Tripp, and Doug Barrick
  Structure-guided rational design of the substrate specificity and catalytic activity of an enzyme / Jung Min Choi and Hak-Sung Kim
  Biological fitness landscapes by deep mutational scanning / Jacob D. Mehlhoff and Marc Ostermeier
  Focused rational iterative site-specific mutagenesis (FRISM) / Danyang Li, Qi Wu, and Manfred T. Reetz
  Statistical analysis of mutational epistasis to reveal intramolecular interaction networks in proteins / Charlotte M. Miton, John Z. Chen, Kalum Ost, Dave W. Anderson, and Nobuhiko Tokuriki
  Machine learning-assisted enzyme engineering / Niklas E. Siedhoff, Ulrich Schwaneberg, and Mehdi D. Davari
  Ultrahigh throughput screening for enzyme function in droplets / Stefanie Neun, Paul J. Zurek, Tomasz S. Kaminski, and Florian Hollfelder.

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• Article

  Comparision of low dose heparin and low dose heparin combined with aspirin in prevention of deep vein thrombosis after total hip replacement.

  Flicoteaux H, Kher A, Jean N, Blery M, Judet T, Honnart F, Pasteyer J.

  Pathol Biol (Paris). 1977 Dec;25 Suppl:55-8.

  In a prospective randomised trial, two groups of 20 patients each were compared. The first group received low dose heparin (t.i.d.) and the second group received low dose heparin (t.i.d.) combined with aspirin. The incidence of deep vein thrombosis (DVT) were determined using both radioactive fibrinogen uptake test and venography. The correlation between the two methods of diagnosis was better than 90%. There was no significant difference in the incidence of DVT between the two prophylactic regimen. A significant tendancy towards increased bleeding in observed with the combination of low dose heparin and aspirin.

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  PubMed