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  • Book
    Michele Fimiani, Pietro Rubegni, Elisa Cinotti, editors.
    Summary: This book provides a complete overview on the latest available technologies in dermatology, while discussing future trends of this ever-growing field. This handy guide provides clinicians and researchers with a clear understanding of the advantages and challenges of laser and imaging technologies in skin medicine today. It also includes a section on imaging techniques for the evaluation of skin tumors, with chapters devoted to dermoscopy, in vivo and ex vivo reflectance confocal microscopy, high frequency ultrasound, optical coherence tomography, and a closing part on latest approaches to wound management. Completed by over 200 clinical images, Current Technology in Practical Dermatology: Non-Invasive Imaging, Lasers and Ulcer Management is both a valuable tool for the inpatient dermatologist and for physicians, residents, and medical students in the field.

    Contents:
    Foreword
    Preface
    Section I
    Imaging techniques for the evaluation of skin diseases
    1. Dermoscopy: fundamentals and technology advances
    2. Dermoscopy for benign melanocytic skin tumors
    3. Dermoscopy for Melanoma
    4. Dermoscopy for non-melanocytic benign skin tumors
    5. Demoscopy for non-melanocytic malignant skin tumors
    6. Dermoscopy for inflammatory diseases
    7. Dermoscopy for infectious diseases
    8. Digital dermoscopy analysis
    9. Optical super-high magnification dermoscopy
    10. Fluorescence videodermoscopy
    11.Total body photography and sequential digital dermoscopy for melanoma diagnosis
    12. History and Fundamentals of Reflectance Confocal Microscopy
    13.In vivo reflectance confocal microscopy for benign melanocytic skin tumors
    14. In vivo reflectance confocal microscopy for melanoma
    15. In vivo reflectance confocal microscopy for non melanocytic benign skin tumors
    16. In vivo reflectance confocal microscopy for non melanocytic malignant skin tumours
    17. In vivo Reflectance Confocale Microscopy for Inflammatory Diseases
    18. In vivo reflectance confocal microscopy for infectious diseases
    19. In vivo reflectance confocal microscopy for mucous membranes
    20. Ex vivo confocal microscopy
    21. Ultrasound
    22. Optical coherence tomography
    23. High-Definition optical coherence tomography
    24. 3D imaging
    25. Raman spectroscopy
    26. Multispectral and Hyperspectral Imaging for skin acquisition and analysis
    27. Electrical impedance in dermatology
    Section II
    Lasers and light sources technologies in dermatology
    28. Laser Light and Light-tissue Interaction
    29. Laser and light sources: safety and organization issues
    30. Intense polichromatic lights and light emitting diodes:what's new
    31. Vascular lasers: tips and protocols
    32. Broadband intense pulsed lights for vascular malformations
    33. Pigment specific lasers for benign skin lesions and tattoos: long pulsed, nanosecond and picos econd lasers
    34. Skin resurfacing: ablative and non-ablative lasers
    35. Photorejuvenation: concepts, practice, perspectives
    36. Laser hair removal: updates
    37. Biophotonic therapy induced photobiomodulation
    38. Photodynamic Therapy
    Section III
    Technological advances in wound management
    39. Temporary dressing
    40. Extracellular matrices
    41. Skin bank bioproducts: the basics
    42. Clinical applications of skin bank bioproducts
    43. Negative Pressure Wound Therapy
    44. Tissue Engineered skin substitutes
    45. Biologics in Wound Management
    46. Stem Cell in Wound Healing
    Section IV
    New complementary tools for dermatologic diagnosis
    47. Microbiopsy in dermatology
    48. Noninvasive genetic testing: adhesive patch-based skin biopsy and buccal swab
    49. Liquid biospsies.
    Digital Access Springer 2020
  • Article
    Hagen FS, Young ET.
    J Virol. 1978 Jun;26(3):783-92.
    The size of lysozyme mRNA from T7-infected E. coli RNase III+ and RNase III- strains was analyzed by sucrose gradient sedimentation, dimethylsulfoxide (Me2SO) sucorse gradient sedimentation, and preparative gel electrophoresis. Each technique revealed a similar size distribution of multiple lysozyme mRNA's. Analysis by preparative gel electrophoresis of RNA extracted after infection of Escherichia coli Bst (RNase III+) separated lysozyme mRNA into six peaks of activity ranging in size from 0.2 x 10(6) to 1.9 x 10(6) daltons. Four well-resolved major peaks of activity were detected, having apparent molecular weights of approximately 0.61 x 10(6), 0.76 x 10(6), 0.92 x 10(6), and 1.3 x 10(6). A broad band of activity, with a molecular weight range from 0.2 x 10(6) to 0.37 x 10(6), was also present, and a sixth peak of activity was sometimes observed that migrates with a mobility corresponding to a molecular weight of 1.9 x 10(6). Judging from their molecular weight as estimated by electrophoresis, most, if not all, of the lysozyme mRNA's were polycistronic. The RNA extracted after infection of an RNase III- host contained a more heterogeneous collection of lysozyme mRNA's. In addition to lysozyme mRNA activity on RNAs with molecular weights between 0.2 x 10(6) and 1.9 x 10(6), RNA species with molecular weights estimated at 4 x 10(6) to 5 x 10(6) were also detected. The data indicate that RNase III processes at least some of the primary lysozyme transcripts. The multiple lysozyme mRNA's represent discrete RNA species rather than aggregates because analysis of the size of lysozyme mRNA under completely denaturing conditions, in Me2SO, produced a similar size distribution of lysozyme mRNAs. Also, treatment of RNA with 90% Me2SO, which separates the strands of a completely double-stranded RNA, did not significantly alter the electrophoretic mobility of the lysozyme mRNA.
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