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  • Book
    Donald M. Broom, Ken G. Johnson.
    Contents:
    Intro; Animal Welfare Series Preface; Preface to Second Edition; Acknowledgements; Contents;
    Chapter 1: One Welfare, One Health, One Stress: Humans and Other Animals; 1.1 The Terms Animal, Welfare, Health and Stress; 1.2 Animal Welfare and Social Change; 1.3 The Debate About Animal Usage; 1.4 Genetics, Epigenetics and What the Environment Can Change; 1.5 The Challenge Ahead; References;
    Chapter 2: Adaptation, Regulation, Sentience and Brain Control; 2.1 Adaptation; 2.2 Homeostatic Control; 2.3 Sentience and the Role of the Brain in Coping; 2.4 Habituation and Sensitisation 2.10 Other Feelings and Emotions: Positive and Negative2.11 Development of Regulatory Systems; 2.11.1 Early Abilities, Preferences and Experiences; 2.11.2 Learning and Memory; 2.11.3 Lifetime and Evolutionary Changes; References;
    Chapter 3: Limits to Adaptation; 3.1 Limitations of Timing and Temporal Aspects of Stimulus Modality; 3.1.1 Changes in Frequency; 3.1.2 Changes in Duration; 3.1.3 The Impact of Novelty; 3.1.4 The Value of Forewarning; 3.2 Limitations of Intensity as an Information Basis for Adaptation; 3.2.1 Changes in Intensity; 3.2.2 Hazard Avoidance and Lethal Limits 2.5 Motivational State2.6 Outputs from Decision Centres; 2.6.1 Neural and Muscular Outputs; 2.6.2 Hormonal and Neurohormonal Outputs; 2.7 Control Systems and Needs; 2.7.1 Simple Models of Control; 2.7.2 Motivational State as the Determinant of Action; 2.7.3 Other Concepts that Have Been Used to Explain Motivation; 2.7.4 The Concepts of Needs and Freedoms; 2.7.5 Motivational Dilemmas and the `Trade-off ́Concept; 2.8 Types of Control; 2.8.1 Rates of Neural and Hormonal Response; 2.8.2 Feedback and Feedforward Controls; 2.8.3 Predictability of Stimulation; 2.9 Pain 3.3 Variation in Adaptation Has Consequences for Responses to Stimulation3.4 Integrating Time, Intensity and Mode of Stimulation; 3.5 The Concepts of Tolerance and Coping; 3.6 Variations in Patterns of Adaptation; 3.6.1 Differing Rates and Methods of Adaptation; 3.6.2 Hypersensitivity; 3.6.3 Hyposensitivity and Stress-Induced Analgesia; 3.7 Other Factors Affecting Adaptation and Coping; 3.7.1 Lack of Stimulation; 3.7.2 Unpredictable Stimulation; 3.7.3 Frustration of Behavioural Output; 3.8 Effects of Human Selection of Animals on Their Ability to Adapt; References
    Chapter 4: Stress and Welfare: History and Usage of Concepts4.1 Stress; 4.2 Welfare; 4.3 Welfare Assessment; 4.4 Welfare in Relation to Stress; 4.5 Welfare in Relation to Naturalness; 4.6 Welfare and Well-Being; 4.7 Welfare and Quality of Life; 4.8 Welfare and a Life Worth Living; 4.9 Welfare in Other Languages; 4.10 Welfare and Sentience; References;
    Chapter 5: Assessing Welfare: Short-Term Responses; 5.1 Behavioural Measures of Welfare; 5.1.1 Orientation and Startle Responses; 5.1.2 Individual Differences in Behavioural Responses; 5.1.3 Measures for Assessing Pain
    Digital Access Springer 2020
  • Article
    Vesterberg O, Hansén L.
    Biochim Biophys Acta. 1978 Jun 21;534(2):369-73.
    Proteins in aqueous salt solutions (up to 4 mil) were adsorbed by hydrophobic interaction on phenyl-Sepharose gel (0.1 ml) in small columns. After washing out excess salt, gels were applied on the surface of flat bed polyacrylamide gels for isoelectric focusing, which resulted in efficient desorption and transport of protein out of the phenyl-Sepharose gel. There was no difficulty in obtaining a fifty-fold concentration. The following parameters at adsorption of protein were studied: (i) salt concentration in the protein solution; (ii) phenyl-Sepharose gel adsorptive capacity for protein; (iii) suitable volume of washing solution for the phenyl-Sepharose gel. Theoretical aspects on factors promoting adsorption and desorption of proteins on phenyl-Sepharose gel are discussed. Also discussed are earlier used procedures for concentration and/or dialysis. When dilute protein solutions are to be examined for analytical purposes, the proposed procedures seems to be a valuable aid, which does not require expensive equipment, and is quick and simple to perform.
    Digital Access Access Options