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  • Book
    M. Tofazzal Islam, M. Mahfuz Rahman, Piyush Pandey, Michael Henry Boehme, Geert Haesaert, editors.
    Summary: This book provides a comprehensive resource for researchers and students involved with studying the roles of Bacilli in sustainable crop production technologies. Chapters included in the book not only elaborate on beneficial traits of Bacilli, but also highlight utilization of these microbes for producing industrially important antibiotics, enzymes, probiotics and other useful biochemicals. Feeding ever increasing world population from shrinking arable acreage led to a synthetic input (fertilizer, growth regulator, pesticide etc.) based crop production system, which has been creating both environmental and health hazards and may also make the whole production system unsustainable. Researchers, food producers and consumers alike, now realize that this world needs effective, environmentally smart agricultural technologies that are safe for people use less synthetic inputs and protect natural resources. To overcome the challenge of increasing food production with a significant reduction of agrochemicals use, a great deal of interest and research have been devoted to beneficial microorganisms/biostimulants in recent days with noteworthy positive results. Bacillus based biopesticide together with other bio-rational approaches may play a critical role in helping all the key drivers of sustainable, environmentally responsible food production with enhanced food quality. Although multiple strains of Bacillus spp. showed promise to contribute to the sustainable agriculture by making nutrients available to plants, providing additional defense to adverse abiotic and biotic stresses, research on Bacillus based product formulation, rate and crop selection, optimization of application timing are urgently needed. As crop plants are more vulnerable to abiotic and biotic stresses at the seedling stage, optimization of application timing should aid in alleviating those stresses for the successful completion of life cycle of a plant.

    Contents:
    1. Management of fungal diseases on cucumber (Cucumis sativus L.) and tomato (Solanum lycopersicum L.) crops in greenhouses using Bacillus subtilis
    2. Bacillus species: A potential plant growth regulator
    3. Bacilli in the Biocontrol of Mycotoxins
    4. Bacillus subtilis and its effect on the post-harvest of fruit and flowers
    5. Plant growth-promotion by ACC deaminase-producing Bacilli under salt stress conditions
    6. Bacillus subtilis-mediated Abiotic Stress Tolerance in Plant
    7. Exploring the Utility of Aneurinibacillus as a Bioinoculant for Sustainable Crop Production and Environmental Applications
    8. Phylogeny and Taxonomy of agriculturally important Bacillus species
    9. Endophytic Bacillus species induced systemic resistance to plant diseases
    10. Genomics and Post-genomics Approaches for Elucidating Molecular Mechanisms of Plant Growth Promoting Bacilli
    11. Tapping the Potential of Metabolomics in New Natural Products Discovery from Bacillus Species
    12. Genomic insights and comparative genomics of Bacillus species having diverse mechanisms of biocontrol against fungal phytopathogens
    13. Bacillus species as biocontrol agents for fungal plant pathogens
    14. Application Method and Efficacy of Bacillus spp in Mitigating Abiotic and Biotic Stresses and Enhancing Plant Performance
    15. Bacillus thuringiensis-based Gene Pyramiding a way Forward for a Combined Horizontal and Vertical Resistance in Plant
    16. Probiotic Bacilli in Sustainable Aquaculture.
    Digital Access Springer 2019
  • Article
    Miller JS, Burgess RR.
    Biochemistry. 1978 May 30;17(11):2054-9.
    A method for the rapid and quantitative analysis of 5'-terminal oligonucleotides of RNAs made in vitro is described. The method involves synthesis of RNA in the presence of [gamma-32P]ATP or GTP, isolation of the RNA, and digestion with T1 or pancreatic ribonucleases to release labeled 5'-triphosphate termanated oligonucleotides. The oligonucleotides are then subjected to chromatography on a polyethyleniminecellulose thin-layer system using 2 M LiCl, 0.01 M EDTA (pH 6.5) in the first dimension and 1.5 M LiCl, 1.8 M formic acid, 0.005 M EDTA (pH 2.0) in the second. RNAs made with E. coli RNA polymerase and lambdacb2, T7, T4, and adenovirus 2 DNA yield characteristic fingerprint patterns. The utility of this method in studying selectivity of in vitro RNA chain initiation is discussed.
    Digital Access Access Options