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  • Book
    Malali Gowda, Ambardar Sheetal, Chittaranjan Kole, editors.
    Summary: This book describes the sequencing efforts for Neem (Azadirachta indica A. Juss), one of the most versatile tropical evergreen tree species. The neem tree is a source of various natural products, including the potent biopesticide azadirachtin and limonoids, which have a broad spectrum of activity against insect pests and microbial pathogens. To identify genes and pathways in neem, three neem genomes and several transcriptomes are studied using next-generation sequencing technologies. Neem has been extensively used in Ayurveda, Unani and homoeopathic medicine and is often referred to as the 'village pharmacy by natives due to its wealth of medicinal properties. Besides the description of the genome, this book discusses the neem microbiome and its role in the production of neem metabolites like salanin, nimbin and exopy-azadirachtin under in vitro conditions. It also highlights cell and tissue culture using various neem explants including the leaf, root, shoot, cambium, etc.

    Economic/Academic importance
    Botanical descriptions and distribution
    Traditional practices and knowledge of neem
    Biochemistry, medicinal properties and molecular biology of neem bioactives
    Quantification of secondary metabolites of neem
    Utilization of neem and neem products in agriculture
    Requirement of whole-genome sequencing
    Strategies & tools for sequencing
    Genome Assembly
    Repetitive sequences
    Gene annotation
    Synteny with allied & model genomes
    Comparison of gene families
    Impact on neem tree improvement breeding
    Neem tissue culture
    Neem microbiome
    Future prospects.
    Digital Access Springer 2019