BookDominic W.S. Wong.
Summary: Clear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas. The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning.
Contents:
Fundamentals of Genetic Processes
Introductory Concepts
Structures of Nucleic Acids
Structures of Proteins
The Genetic Process
Organization of Genes
Reading The Nucleotide Sequence of a Gene
Techniques and Strategies of Gene Cloning
Enzymes Used in Cloning
Techniques Used in Cloning
Cloning Vectors for Introducing Genes into Host Cells
Transformation
Isolating Genes for Cloning
Impact of Gene Cloning
Improving Tomato Quality by Antisense Rna
Transgenic Crops Engineered with Insecticidal Activity
Transgenic Crops Conferred with Herbicide Resistance
Growth Enhancement in Transgenic Fish
Impact of Gene Cloning
Microbial Production of Recombinant Human Insulin
Finding Disease-Causing Genes
Human Gene Therapy
Gene Targeting
DNA Typing
Transpharmers
Bioreactors for Pharmaceutical Products
Animal Cloning
Human Genome Sequencing
Manipulating Gene Vector Constructs.