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  • Book
    Manuel Yúfera, editor.
    Summary: This book deals with the fundamentals of key physiological mechanisms involved in the development and growth of fish larvae. Chapters included show how the environmental and nutritional conditions are affecting the developmental process from its molecular basis and how these same conditions also influence the final characteristics of late larvae and fry. This volume provides recent findings on the importance of environmental rhythms, some specific nutrients and the adequate microbial environment in the developmental processes including recent results of current research projects.

    Contents:
    1. Investigating fish larvae-microbe interactions in the 21st century : old questions studied with new tools / Ragnhild I. Vestrum, Birgit Luef, Torunn Forberg, Ingrid Bakke and Olav Vadstein
    2. Environmental cycles and biological rhythms during early development / Francisco Javier Sánchez-Vázquez and José Fernando López-Olmeda
    3. The digestive function in developing fish larvae and fry. From molecular gene expression to enzymatic activity / Manuel Yúfera, Francisco J. Moyano and Gonzalo Martínez-Rodríguez
    4. Variability in digestive enzyme capacity in early stages of marine fish larvae : ontogenetic variations, biorhythms, hormonal control and nutrient sensing mechanisms / Bernd Ueberschär, Carmen Navarro-Guillén, Ana Gomes, Ivar Rønnestad, Carlos Rojas-Garcia, Inken Hanke, Dagh Sommerfeld and Robert Tillner
    5. Phospholipids in marine larval rearing / Keshuai Li, Rolf Erik Olsen, Yang Jin and Yngvar Olsen
    6. Fat-soluble vitamins in fish : a transcriptional tissue-specific crosstalk that remains to be unveiled and characterized / Ignacio Fernández, Paulo Gavaia, Maria J. Darias and Enric Gisbert
    7. Nutritional modulation of marine fish larvae performance / Sofia Engrola, Cláudia Aragão, Luisa M.P. Valente and Luís E.C. Conceição
    8. Fish pigmentation. A key issue for the sustainable development of fish farming / Laura Cal, Paula Suarez-Bregua, Paloma Moran, José Miguel Cerdá-Reverter and Josep Rotllant
    9. Novel aspects of phosphate endocrine control : a key element for the long-term sustainability of finfish aquaculture / Paula Suarez-Bregua, Laura Cal, Pedro M. Guerreiro and Josep Rotllant
    10. Feeding and development of warm water marine fish larvae in early life / Jing Hu, Yibing Liu, Zhenhua Ma and Jian G. Qin.
    Digital Access Springer 2018
  • Article
    Nozawa RT, Yokota T, Kuwahara S.
    J Clin Microbiol. 1978 May;7(5):479-85.
    As Chinese hamster ovary (CHO) cells on plastic proliferate, many cells float off into the medium instead of piling up after they form a monolayer. Fewer cells were floating in the medium when CHO cells were incubated with cholera toxin at a concentration as low as 10 pg/ml. The toxin increased the adhesiveness of the cells forming confluent monolayers so that the floating cells accumulated on the adherent monolayers. On the basis of this finding, a simple, quantitative assay method for cholera and Escherichia coli enterotoxins was devised by cultivating CHO cells in a Linbro multidish and counting the cells in the medium with a Coulter Counter. The method was sensitive enough to detect toxins in 100- to 200-fold-diluted culture media of toxigenic E. coli strains. Little or no activity was detected by this method in the culture medium of nontoxigenic E. coli.
    Digital Access Access Options