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- BookChris Pasero, Margo McCaffery.Summary: This resource in pain management in adults provides numerous reproducible tables, boxes, and figures that can be used in clinical practice and emphasizes the benefits of a multimodal analgesic approach throughout.
Contents:
Neurophysiology of pain and analgesia and the pathophysiology of neuropathic pain
Assessment
Nonopioid analgesics
Opoid analgesics
Adjuvant analgesics.Digital Access ClinicalKey Nursing 2011 - ArticleVisser J, Strating M, van Dongen W.Biochim Biophys Acta. 1978 May 11;524(1):37-44.1. The lifetime of thiamine pyrophosphate-Sepharose 2B affinity matrices synthesized according to Matsuura et al. (Matsuura, A., Iwashina, A. and Nose, Y. (1973) Biochem. Biophys. Res. Commun. 51, 241-246) has been improved. The matrix interacts with bacterial pyruvate dehydrogenase complexes. 2. The synthesis of a stable thiochrome-Sepharose 2B matrix is described. 3. Both matrices bind the pyruvate dehydrogenase complex of Escherichia coli in a 50 mM phosphate buffer, pH 7.0. Elution is possibly by an increase in ionic strength but not by the cofactor or metal-cofactor complexes. 4. The presence of Mg2+, reduces the capacity of the affinity matrices but leads to higher specificity for the multienzyme complex. 5. The pyruvate dehydrogenase complex of E. coli has been successfully purified by combining a classical purification step with these affinity chromatography systems. The method is less suitable for large scale operation.