Search

Search Results

• Book

  Manual of high risk pregnancy & delivery. 5th ed.

  Elizabeth Stepp Gilbert.

  Summary: As the only book of its kind, this text provides a complete resource for care of the high risk patient and her complex needs. It helps you provide the best care and increase positive outcomes with coverage of today's newest technology, physiologic considerations, psychologic implications, health disorders, and other complications in pregnancy. This book also describes how to screen for risk factors, provide preventive management, and intervene appropriately when problems arise. It's a concise, hands-on reference for both inpatient and outpatient settings. --Book Jacket.
  
  Contents:
  Physiologic and nutritional adaptations to pregnancy
  General nursing assessment of the high risk expectant family
  Assessment of fetal well-being
  Perinatal screening, diagnoses, and fetal therapies
  Integrative therapies in pregnancy and childbirth
  Psychologic adaptations
  Perinatal death and bereavement care
  Ethical decision making
  Legal issues and risk management
  Diabetes
  Cardiac disease
  Renal disease
  Autoimmune rheumatic diseases
  Venous thromboembolic disease
  Pulmonary disease and respiratory distress
  Spontaneous abortion
  Ectopic pregnancy
  Gestational trophoblastic disease
  Placental abnormalities
  Disseminated intravascular coagulation
  Hemolytic incompatibility
  Hypertensive disorders
  Preterm labor and multiple gestation
  Premature rupture of membranes
  Trauma
  Sexually and nonsexually transmitted genitourinary infections
  Substance abuse
  Labor stimulation
  Dysfunctional labor
  Prolonged pregnancy.

  Digital Access ClinicalKey Nursing 2011
  Get Shareable Link

  View Details
• Article

  Nonspecific interactions of Escherichia coli RNA polymerase with native and denatured DNA: differences in the binding behavior of core and holoenzyme.

  deHaseth PL, Lohman TM, Burgess RR, Record MT.

  Biochemistry. 1978 May 02;17(9):1612-22.

  We have investigated the nonspecific interactions of Escherichia coli RNA polymerase core and holoenzyme with double-stranded (ds) and single-stranded (ss) DNA. Binding constants for these interactions as functions of such solution variables as monovalent and/or divalent cation concentration, temperature, or pH were determined by the method of deHaseth et a. [deHaseth, P.L., Gross, C.A., Burgess, R.R. and Record, M.T. (1977), Biochemistry 16, 4777--4783] from analysis of the elution of the proteins from small columns containing immobilized DNA. This technique, although as yet empirical, has been demonstrated to yield accurate binding constants fot the nonspecific interation of lac repressor with ds DNA. We find that observed binding constants (Kobsd) are extraordinarily sensitive functions of the monovalent cation concentration for the interactions of both core and holoenzyme with ds DNA. In the absence of divalent cations, the derivatives --(d log Kobsd/d log [Na+]) are 11 +/- 2 for the holo--ds DNA interaction and 21 +/- 3 for the core--ds DNA interaction. Consequently, approximately 11 and 21 low-molecular-weight ions are released, iin the thermodynamic sense, in the formation of the holo--ds and core--ds complexes, respectively (Record, M.T., Jr., Lohman, T.M., and deHaseth, P.L. (1976), J. Mol. Biol. 107, 145--158; Record, M.T., Jr., Anderson, C.F., and Lohman, T.M. (1978), Q. Rev. Biophys., in press). Ion release is a thermodynamic driving force for these nonspecific interactions and causes the stability of the complexes to increase very substantially with a reduction in monovalent ion concnetration. Possible molecular models which account for the different salt sensitivities of the holo--ds and core--ds complexes are discussed. Effects of the competitive ligand Mg2+ on these interactions are also examined. Substantial ion release (approximately 18 monovalent ions) also accompanies the interaction of either holo or core polymerase with ss DNA. Over the range of ion concentrations investigated the holo--ss interaction is substantially stronger than the core--ss interaction; furthermore, we conclude that the interactions of polymerase with ss DNA are, in general, stronger than the nonspecific interations of the enzyme with ds DNA. It is likely that the nonspecific interactions of RNA polymerase with DNA have physiological relevance. Not only is it plausible to assume that the same regions of the protein are involved in both specific and nonspecific interactions, but in addition nonspecific interactions of RNA polymerase and DNA may play role in determining the availability of this protein, in both the thermodynamic and the kinetic sense, for promoter binding and RNA chain initiation [von Hippel. P.H., Revzin, A., Gross, C.A., and Wang, A.C. (1974), Proc. Natl. Acad. Sci U.S.A. 71, 4808--4812]. Consequently, the strong dependences of the nonspecific interactions of RNA polymerase on ionic conditions suggest the possibility of a modulating role of ion concentrations in the control of transcription.

  Digital Access Access Options
  Get Shareable Link

  PubMed