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  • Book
    Tanya L. Fernandez, MS, PA-C, IBCLC, Amy Akerman, MPAS, PA-C.
    Summary: Written by experienced PA educators, this guide details the clinical approach to common presentations such as cough, fever, and skin changes. It also provides a systems-based approach to more than 60 of the most frequently encountered disease entities you will see in this rotation, including dermatitis, pharyngitis, and otitis media. Distinguished by brief, bulleted content with handy tables and figures, the reference offers all pertinent laboratory and imaging studies needed to confirm a diagnosis, with medication and management guidelines. This guide also describes the most common procedures you will learn during the pediatrics rotation, including foreign body removal, reduction of subluxed radial head, and administration of nebulizer treatments.A special chapter on management of urgent pediatric conditions, such as asthma exacerbation, head injuries, and fractures, is also included
  • Article
    Goding JW.
    J Immunol Methods. 1978;20:241-53.
    This brief review summarises the major uses of staphylococcal protein A in immunology. Protein A is covalently linked to the cell wall of most strains of Staphylococcus aureus, and binds immunoglobulin molecules with high affinity. The principal molecule bo-nd is IgG, although in many cases binding is restricted to certain IgG subclasses. Some IgM and IgA binds in certain species. This property allows rapid, simple and economical methods for the purification and analysis of immunoglobulins, and the fractionation of subclasses which are difficult to separate by other means. Fractionation on protein A affinity columns is a simple and efficient way of separating immunoglobulin F (ab) and F (ab')2 from Fc fragments. Intact staphylococci are useful as a solid phase adsorbent for isolating antigen-antibody complexes, membrane antigens and receptors, and to replace 'second antibody' in radioimmunoassay. Finally, protein A has proven useful for the study of antigens and receptors on the surface of intact cells, and for the detection of antibody-secreting cells. Thus, the use of protein A is now the method of choice for many preparative and analytical purposes in immunology.
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