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- ArticleGuimarães MC, Camargo ME, Ferreira AW, Ayres de Castilho E, Nakahara OS.Am J Trop Med Hyg. 1978 Mar;27(2 Pt 1):350-3.By the indirect immunoperoxidase radial immunodiffusion technique the IgG and IgM contents in serum and filter paper eluates were found to remain essentially at equivalent levels for at least 2.5 months after collection.