Bookedited by Jungsan Sohn.
Contents:
Intercellular communication in the innate immune system through the cGAS-STING pathway / Ramya Nandakumar, Samuel Joseph Windross, and Søren R. Paludan
Analysis of human cGAS activity and structure / Wen Zhou, Aaron T. Whiteley, and Philip J. Kranzusch
Chemical synthesis, purification, and characterization of 3′-5′-linked canonical cyclic dinucleotides (CDNs) / Changhao Wang, Min Hao, Qianqian Qi, Yashao Chen, and Jörg S. Hartig
An SPR-based analysis of cGAS substrate KD and steady-state KM values / Erik C. Ralph and Justin Hall
A pyrophosphatase-coupled assay to monitor the NTase activity of cGAS / Richard Hooy and Jungsan Sohn
Tracking the polymerization of DNA sensors and inflammasomes using FRET / Zachary Mazanek and Jungsan Sohn
Monitoring gasdermin pore formation in vitro / Shiyu Xia, Jianbin Ruan, and Hao Wu
Measuring TREX1 and TREX2 exonuclease activities / Wayne O. Hemphill and Fred W. Perrino
Kinetics of DNA-protein association and dissociation by stopped-flow spectroscopy / Johannes Rudolph and Karolin Luger
Characterization of DNA bound cyclic GMP-AMP synthase using atomic force microscopy imaging / Alexander Lushnikov, Richard Hooy, Jungsan Sohn, and Alexey Krasnoslobodtsev
Preparation of filamentous proteins for electron microscopy visualization and reconstruction / Mariusz Matyszewski and Jungsan Sohn
Cryo-EM studies of NAIP-NLRC4 inflammasomes / Nicole Haloupek, Patricia Grob, Jeannette Tenthorey, Russell E. Vance, and Eva Nogales
An integrative protocol for the structure determination of the mouse ASC-PYD filament / Adam Mazur, Petr Broz, and Sebastian Hiller
Protein interactions of the inflammasome adapter ASC by solution NMR / Pedro Diaz-Parga and Eva de Alba
Tracking DNA sensor and inflammasome complexes in cells using confocal microscopy / Brendan Antiochos
Assessment of inflammasome and type I IFN responses to DNA viruses and DNA PAMPS / Guido Massaccesi and Michael A. Chattergoon
Biochemical and microscopic analysis of inflammasome complex formation / Patrycja Swacha, Nelson O. Gekara, and Saskia F. Erttmann
Comet and micronucleus assays for analyzing DNA damage and genome integrity / Hui Jiang, Swarupa Panda, and Nelson O. Gekara
Workflows and considerations for investigating protein interactions of viral DNA sensors / Timothy R. Howard, Bokai Song, and Ileana M. Cristea
TUBE and UbiCRest assays for elucidating polyubiquitin modifications in protein complexes / Swarupa Panda, Hui Jiang, and Nelson O. Gekara.