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  • Book
    editor, Andreas Raabe ; associate editors, Bernhard Meyer, Karl Schaller, Peter Vajkoczy, Peter A. Winkler.
    Summary: "This atlas is a comprehensive compilation of common and frequently used craniotomies and includes all essential details for the training of neurosurgeons. Each craniotomy is described step-by-step in intraoperative color photographs and corresponding drawings, showing all details and landmarks mentioned in the text. The corresponding text gives technical instructions as well as detailed descriptions. The same structure will be used for the description of each craniotomy"--Provided by publisher.

    Contents:
    Basics
    Landmarks
    Convexity Craniotomies
    Midline Craniotomies
    Skull Base Craniotomies
    Skull Base Extensions
    Transsphenoidal Approach
    Decompressive Hemicraniectomy
    Approaches to the Orbita.
    Digital Access
    Provider
    Version
    Thieme-Connect
    2019
    Thieme MedOne Neurosurgery
    2019
  • Article
    Kenny JJ, Merrill JE, Ashman RF.
    J Immunol. 1978 Apr;120(4):1233-9.
    A two-step centrifugation procedure has been developed to isolate greater quantities of highly purified sheep erythrocyte antigen-binding cells (ABC) than previously possible. The first step involves partially separating sheep erythrocyte rosettes from unrosetted lymphocytes by their difference in buoyant density on Ficoll-Hypaque. Subsequent passage through a linear 5 to 10% Ficoll gradient produces further purification of rosettes by sedimentation velocity. Approximately 4.5 X 10(6) ABC can be obtained at 50 to 100% purity from 10(9) immune spleen cells (5 days post-immunization) and 1 X 10(5) ABC at 20 to 40% purity from 10(9) nonimmune spleen cells. The purified ABC from 5-day immune animals are 80 to 90% B cells and 10 to 20% T cells, and represent between 30 and 40% of the original ABC in the spleen cell population. Less than 0.2% of the purified ABC are plaque-forming cells (PFC) and less than 2% have intracellular immunoglobulin (Ig) or J chain. The quantities of ABC obtained are sufficient for investigating biochemical parameters of antigen-induced lymphocyte activation and for direct analysis of the surface isotypes found on antigen-binding cells after immunization.
    Digital Access Access Options