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  • Book
    edited by Jonathan Thompson, Iain Moppett, Matthew Wiles.
    Contents:
    Section 1: Basic sciences. General principles of pharmacology
    Data, statistics and clinical trials
    Inhalational anaesthetics and medical gases
    Intravenous anaesthetics and sedatives
    Local anaesthetics
    Pain
    Nausea and vomiting
    Muscle function and neuromuscular blockade
    Cardiovascular system
    Respiratory system
    Renal system
    Fluid, electrolyte and acid-base balance
    Metabolism, the stress response and thermoregulation
    Blood, coagulation and transfusion
    Section 2: Physics and apparatus. Basic physics
    Equipment
    Clinical measurement and monitoring
    Section 3: Fundamentals of anaesthesia & perioperative medicine. Quality and safety
    Preoperative assessment
    Coexisting disease
    Consent and information
    The practical conduct of anaesthesia
    Airway management
    Pain management
    Local and regional anaesthesia
    Complications
    Management of critical incidents
    Resuscitation
    Postoperative care
    Section 4: Clinical anaesthesia. The high-risk surgical patient
    The older surgical patient
    The obese patient
    Paediatric anaesthesia
    Day surgery
    General, gynaecological and genitourinary surgery
    Orthopaedic surgery
    ENT, maxillofacial and dental surgery
    Ophthalmic surgery
    Vascular, endocrine and plastic surgery
    Neurosurgery
    Thoracic surgery
    Cardiac surgery
    Obstetric anaesthesia and analgesia
    Emergency and trauma surgery
    Anaesthesia in low and middle-income areas
    Anaesthesia outside the operating theatre
    Organ transplantation
    Intensive care medicine.
    Digital Access ClinicalKey 2019
  • Article
    Taketani S, Nagai J, Katsuki H.
    Biochim Biophys Acta. 1978 Mar 30;528(3):416-23.
    For extraction of free and esterified sterols from yeast cells, a method was devised in which both forms of sterols were extracted with light petroleum after the treatment of the cells with acetone, and then with dimethylsulfoxide. The content of sterol esters in the cells under aerobic conditions markedly increased with time, amounting to 95% of the total sterols under some conditions. However, the formed sterol esters were decreased, accompanied with an increase of free sterols, when the cells were put under anaerobic conditions. Variations of radioactivities of both sterols which had been labeled in the side chain by incubation of the cells with [Me[-14C]methionine were examined on the cells grown under various conditions. No variation was observed on the cells under aerobic conditions. On the other hand, the labeled esters were hydrolyzed to yield free sterols in the cells under anaerobic conditions. In the cells under aerobic conditions, the free sterols were found to consist mainly of ergosterol, whereas the esterified sterols contained considerable amounts of zymosterol, lanosterol, and other intermediate sterols besides ergosterol.
    Digital Access Access Options