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- ArticleHobbs KM, Sole E, Bettelheim KA.Zentralbl Bakteriol Orig A. 1977 Nov;239(3):409-13.Bipolar staining of the trophozoites of Toxoplasma gondii has been observed with a number of sera from patients. It has been demonstrated that the serum factors involved are IgM. The majority of sera demonstrating this phenomenon had raised nonspecific IgM levels.