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- Bookedited by Christine Ziegler.Summary: New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET on transporters, Dynamics of channels and transporters investigated by NMR, Structure-function studies on channels and transporters, and a section on MemStar, a new GFP-based expression and purification system for transporters and channels.
Contents:
Chapter 1: Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM / Rouslan Efremov, Christos Gatsogiannis and Stefan Raunser
Chapter 2: SSM-Based Electrophysiology for Transporter Research / Andre Bazzone, Maria Barthmes and Klaus Fendler
Chapter 3: Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters / Joseph Lyons, Andreas Bøggild, Poul Nissen and Jens Frauenfeld
Chapter 4: Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy / Joris M.H. Goudsmits, Antoine M. van Oijen and Dirk J. Slotboom
Chapter 5: The MEMbrane Protein Single ShoT Amplification Recipe: MemStar / Aziz Abdul-Qureshi, Pascal F. Meier, Chiara Lee and David Drew
Chapter 6: Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli / Jacopo Marino, Katharina Holzhüter, Benedikt Kuhn and Eric R. Geertsma
Chapter 7: Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling / Christopher Mulligan and Joseph A. Mindell
Chapter 8: Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR / Christos Pliotas
Chapter 9: Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein / Annette Strege, Byron Carpenter, Patricia C. Edwards and Christopher G. Tate.Digital Access ScienceDirect 2017 - ArticlePeters RK, Benson H, Porter D.Am J Public Health. 1977 Oct;67(10):946-53.An experiment conducted at the corporate offices of a manufacturing firm investigated the effects of daily relaxation breaks on five self-reported measures of health, performance, and well-being. For 12 weeks, 126 volunteers filled out daily records and reported bi-weekly for additional measurements. After four weeks of baseline monitoring, they were divided randomly into three groups: Group A was taught a technique for producing the relaxation response; Group B was instructed to sit quiety; Group C received no instructions. Groups A and B were asked to take two 15-minute relaxation breaks daily. After an eight-week experimental period, the greatest mean improvements on every index occurred in Group A; the least improvements occurred in Group C; Group B was intermediate. Differences between the mean changes in Groups A vs C reached statistical significance (p < 0.05) on four of the five indices: Symptoms, Illness Days, Performance, and Sociability-Satisfaction. Improvements on the Happiness-Unhappiness Index were not significantly different among the three groups. The relationship between amount of change and rate of practicing the relaxation response was different for the different indices. While less than three practice periods per week produced little change on any index, two daily sessions appeared to be more practice than was necessary for many individuals to achieve positive changes. Somatic symptoms and performance responded with less practice of the relaxation response than did behavioral symptoms and measures of well-being. (Am. J. Public Health 67:946-953,1977)