ArticleKislina OS, Naroditskiĭ BS, Chaplygina NM, Kamarov EV, Tikhonenko TI.
Vopr Virusol. 1977 May-Jun(3):271-4.
Optimal conditions for ligation of simian adenovirus type 7 (SA-7) DNA fragments formed under the effect of treatment of the intact molecule with Eco RI endonuclease were established. It was shown that up to 30% of the original material may be ligated and transferred into a structure with molecular weight 23 X 10(6) daltons which corresponded to the molecular weight of the intact SA-7 DNA. The data of the existence of one recognition site for Eco RI in SA-7 DNA were confirmed. The biological activity of the ligated material was demonstrated on lambda-III phage DNA.