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- Bookedited by Douglas K. Pleskow, Chief, Clinical Gastroenterology, ... Show More Beth Israel Deaconess Medical Center, Associate Clinical Professor of Medicine, Harvard Medical School, Boston, MA, United States, Tolga Erim, Director of Endoscopy, Department of Gastrenterology, Digestive Disease Center, Cleveland Clinic Florida, Weston, FL, United States.Digital Access ClinicalKey 2016
- ArticleVerheugt FW, von dem Borne AE, Décary F, Engelfriet CP.Br J Haematol. 1977 Aug;36(4):533-44.An indirect immunofluorescence technique for the detection of alloantibodies against granulocytes was developed. A major problem was the unspecific fluorescence of granulocytes, due to unspecific adherence of immunoglobulins to the cell membrane. This could be suppressed by the prefixation of the granulocytes with paraformaldehyde. The developed test proved to be specific and sensitive and to have a high reproducibility. Strongly positive reactions were obtained with antisera containing granulocyte-specific agglutinins or granulocyte-cytotoxins, but also with some sera that did not react in either the agglutination or the cytotoxicity test. HLA antisera also gave positive reactions, but strong anti-A, anti-B or anti-D sera did not react. With this technique leucocyte antibodies can be detected in a higher percentage of patients with febrile transfusion reactions.