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  • Book
    edited by Amalia Cochran, Ruth Braga.
    Contents:
    The OR as a study in cultural anthropology / Amalia Cochran
    From start to finish : understanding how we get here / Ruth Braga
    The myth of the kind surgeon / Thomas K. Varghese Jr.
    Who are these people?
    Teamwork and communication in the operating room / Louise Hull and Nick Sevdalis
    The OR and humor / Ruth Braga
    Personalities in the operating room / Ross M. Blagg
    2015 Grammy awards category : music in the operating room / Marie Crandall
    Welcome to the jungle : the five senses of the operating room / Elizabeth Hanes
    Operating room equipment from A to Z / Diane Tyler and Ruth Braga
    Robot basics-the nurses' perspective / Sebrena Banecker
    My robot experience, or how surgeons learn new techniques / Walter Medlin
    Dancing around the room
    The OR and the surgical field / Karen Porter
    Lines and tubes : what are all of those things sticking out of the patient's body? / Halle Kogan and Thaona D. Garber
    Positioning the patient / Karen Porter
    Patient safety in the OR / Jon Worthen
    Distractions and interruptions in the operating room / Nick Sevdalis and Louise Hull
    Intraoperative photos
    Notes from your attending / Lawrence A. Shirley and Christian Jones
    Tomfoolery, shenanigans, and hazing in surgery / Luke V. Selby
    Privacy
    keep it to yourself! / Ruth Braga
    Keep calm and trust the count / Cynthia Howard.
    Digital Access AccessSurgery 2017
  • Article
    Goldberg RB, Geremia R, Bruce WR.
    Differentiation. 1977;7(3):167-80.
    Separation of labelled nuclei by sedimentation velocity at unit gravity (Staput method) was used to study the timing of histone synthesis and replacement by testis-specific basic nuclear protein (TSP) during spermatogenesis in the mouse. Animals were injected (intratesticularly) with 1.25 micronCi per testis 3H-arginine or 2.5 micronCi per testis 3H-lysine, testis nuclei were separated, and the acid extract of each nuclear fraction was analyzed by acrylamide gel electrophoresis. The distribution of labelled histones and TSP in separated nuclei was assessed 2 h after incorporation. Changes in the labelled histone and TSP content of nuclei during subsequent differentiation (1--34 days post-label) was followed in fractions of separated testis cell nuclei and in nuclei of cauda epididymal spermatozoa. Analysis of total histone and (TSP) content indicated quantitative changes during development. Nuclei from primary spermatocytes had relatively larger amounts of histones H1 and H4. Spermatid nuclei showed a relative reduction in histones H1 and H4, coincident with the appearance of TSP in these nuclei. These results suggested that synthesis and/or removal of certain histones must occur in late primary spermatocyte and early spermatid stages of spermatogenesis. Results of labelling experiments indicated several periods of histone synthesis during spermatogenesis: (1) closely associated with the last DNA synthesis(i.e., in early primary spermatocytes), (2) late in meiotic prophase (i.e., in pachytene primary spermatocytes) and (3) simultaneous with TSP synthesis (i.e., in late spermatids). Histone H1 was more heavily labelled toward the end of the primary spermatocyte period. Histone H4 was more heavily labelled in the early primary spermatocyte period, and again at the time of TSP synthesis in spermatids. Histones synthesized before the pachytene primary spermatocyte stage appeared to be replace, but histones synthesized later in spermatogenesis appeared to be at least partially retained in epididymal spermatozoa. These results suggested that repeated specific alterations in the protein complement of the nucleus are an integral part of spermatogenic differentiation in the mouse.
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