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  • Book
    edited by Francois Lacour-Gayet, Edward L. Bove, Viktor Hraška, Victor O. Morell, Thomas L. Spray
    Summary: This book addresses the most technically demanding cardiac surgical techniques, many of which are often performed on small infants. Conotruncal heart defects are a group of congenital anomalies of the cardiovascular system that are a leading cause of symptomatic cyanotic cardiac disease diagnosed in utero. Surgery of Conotruncal Anomalies provides a wealth of practical information and has been written by a collection of world renowned experts. They discuss the most challenging but life-changing surgeries which, if performed perfectly, allow the child to lead a normal adult life in the majority of cases. This book and the large number of truly stunning explanatory illustrations contained within is an essential resource for junior surgeons as well as trainees and practicing cardiac surgeons, pediatric cardiologists and surgeons, and cardiac imagers, to be used as a critical reference when performing these complex cases.
    Digital Access Springer 2016
  • Article
    Gnedoĭ SN, Babushkina LM, Frolov VN, Levashev VS.
    Zh Mikrobiol Epidemiol Immunobiol. 1977(1):99-104.
    Preparations of DNA of R6K plasmide obtained by various methods on the basis of the "clarified" lysate: by gel filtration on sepharose 4B, centrifugation in cesium chloride with ethidium bromide gradient, were analysed in the E. coli C600. S. typhimurium AG37, Pr. vulgaris 4636, S. marcescens 20-10 transformation. The frequency of transformation proved to depend on the extent of DNA purification. Factors influencing the E. coli C600 competence and the transformation efficacy (the phase of the culture growth, the concentration of cells in the mixture with DNA, theCaCl2 concentration, the time of the cell incubation at 42 degrees C) were studied. Kinetics of the phenotypical expression of the plasmide signs of ampicillin and streptomycin resistance in transformation was also studied in this work.
    Digital Access Access Options