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- Bookedited by Alberto Sanchez-Diaz, Pilar Perez.Contents:
Time-lapse fluorescence microscopy of budding yeast cells / Arun Kumar and Manuel Mendoza
Real-time visualization and quantification of contractile ring proteins in single living cells / Reshma Davidson ... [et al.]
Fluorescence recovery after photo-bleaching (FRAP) and fluorescence loss in photo-bleaching (FLIP) experiments to study protein dynamics during budding yeast cell division / Alessio Bolognesi ... [et al.]
High-speed super-resolution imaging of live fission yeast cells / Caroline Laplante ... [et al.]
Monitoring chitin deposition during septum assembly in budding yeast / Irene Arcones and Cesar Roncero
Imaging septum formation by fluorescence microscopy / Juan Carlos Ribas and Juan Carlos G. Cortés
Visualization of cytokinesis events in budding yeast by transmission electron microscopy / Franz Meitinger and Gislene Pereira
Visualization of fission yeast cells by transmission electron microscopy / Matthias Sipiczki
Characterization of septin ultrastructure in budding yeast using electron tomography / Aurélie Bertin and Eva Nogales
Isolation of cytokinetic actomyosin rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe / Junqi Huang ... [et al.]
Measurements of myosin-II motor activity during cytokinesis in fission yeast / Qing Tang, Luther W. Pollard, and Matthew Lord
In vitro biochemical characterization of cytokinesis actin-binding proteins / Dennis Zimmermann ... [et al.]
Characterization of cytokinetic F-BARs and other membrane-binding proteins / Nathan A. McDonald and Kathleen L. Gould
Analysis of three-dimensional structures of exocyst components / Johannes Lesigang and Gang Dong
Analysis of Rho-GTPase activity during budding yeast cytokinesis / Masayuki Onishi and John R. Pringle
Detection of phosphorylation status of cytokinetic components / Franz Meitinger, Saravanan Palani, and Gislene Pereira
Studying protein-protein interactions in budding yeast using co-immunoprecipitation / Magdalena Foltman and Alberto Sanchez-Diaz
Conditional budding yeast mutants with temperature-sensitive and auxin-inducible degrons for screening of suppressor genes / Asli Devrekanli and Masato T. Kanemaki
Synchronization of the budding yeast Saccharomyces cerevisiae / Magdalena Foltman, Iago Molist, and Alberto Sanchez-Diaz
Fission yeast cell cycle synchronization methods / Marta Tormos-Pérez, Livia Pérez-Hidalgo, and Sergio Moreno
Review of fluorescent proteins for use in yeast / Maja Bialecka-Fornal, Tatyana Makushok, and Susanne M. Rafelski
Visualization and image analysis of yeast cells / Steve Bagley
Toolbox for protein structure prediction / Daniel Barry Roche and Liam James McGuffin
From structure to function : a comprehensive compendium of tools to unveil protein domains and understand their role in cytokinesis / Sergio A. Rincon and Anne Paoletti.Digital Access Springer 2016 - ArticleLoest RA.Comp Biochem Physiol B. 1979;63(1):103-7.1. Urease from a sea urchin Lytechinus variegatus, was purified 300-fold, using heat precipitation, ethanol precipitation and gel filtration. 2. The pH optimum is 8.0. 3. The apparent Michaelis constant for urea is 0.13 mM at pH 8.0. 4. The inhibitory effects of seven reagents on urease were evaluated. The pattern of inhibition is similar to other invertebrate ureases. 5. L. variegatus urease is compared with that of several other invertebrates, and its possible significance in CaCO3 formation is discussed.