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- Bookedited by Hicham Zegzouti and Said A. Goueli.Contents:
HTRF kinase assay development and methods in inhibitor characterization / Yong Jia, Mari Manuia, and Jose Juarez
Application of eukaryotic elongation factor-2 kinase (eEF-2K) for cancer therapy : expression, purification, and high-throughput inhibitor screening / Clint D. J. Tavares ... [et al.]
Recombinant kinase production and fragment screening by NMR spectroscopy / Byeonggu Han and Hee-Chul Ahn
Bioluminescence methods for assaying kinases in quantitative high-throughput screening (qHTS) format applied to yes1 tyrosine kinase, glucokinase, and PI5P4Kα lipid kinase / Mindy I. Davis, Douglas S. Auld, and James Inglese
Using bioluminescent kinase profiling strips to identify kinase inhibitor selectivity and promiscuity / Hicham Zegzouti, Jacquelyn Hennek, and Said A. Goueli
Measuring activity of phosphoinositide lipid kinases using a bioluminescent ADP-detecting assay / Andrew W. Tai and Jolanta Vidugiriene
High-throughput radiometric kinase assay / Krisna C. Duong-Ly and Jeffrey R. Peterson
High-content assay to screen for modulators of EGFR function / Christophe Antczak and Hakim Djaballah
Monitoring protein kinase expression and phosphorylation in cell lysates with antibody microarrays / Hong Zhang, Xiaoqing Shi, and Steven Pelech
From enzyme to whole blood : sequential screening procedure for identification and evaluation of p38 MAPK inhibitors / Silke M. Bauer ... [e tal.]
Genetically encoded fluorescent indicators to visualize protein phosphorylation in living cells / Moritoshi Sato and Yoshio Umezawa
Characterization of an engineered Src kinase to study Src signaling and biology / Leanna R. Gentry .. [et al.]
Screening one-bead-one-compound peptide libraries for optimal kinase substrates / Thi B. Trinh and Dehua Pei
Determination of the substrate specificity of protein kinases with peptide micro- and macroarrays / Shenshen Lai ... [et al.]
Rapid identification of protein kinase phosphorylation site motifs using combinatorial peptide libraries / Chad J. Miller and Benjamin E. Turk.Digital Access Springer 2016 - ArticleSly MR, Walker DG.Comp Biochem Physiol B. 1978;61(3):471-7.1. A method for the preparation of hepatocytes from livers of 11-15-day old rats is described. These cells in general behave similarly to hepatocytes made from adult rats with respect to stimulation of gluconeogenesis by glucagon and adrenaline and the effects of added oleate. 2. Significant differences in the behaviour of hepatocytes from neonatal and adult rats were nevertheless seen in certain situations, e.g. with alanine as gluconeogenic substrate, and appeared to be related to the redox state of the cells. 3. The importance of redox state upon gluconeogenesis was examined in more detail by determining the effects of oleate, ethanol and DL-3-hydroxybutyrate alone and in combinations. Major differences between neonatal and adult hepatocytes were again observed with alanine as substrate. 4. A discussion concludes that, while some relevant differences in the enzyme complements of neonatal and adult rat livers are known, it is the high capacity of the neonatal liver to generate reducing power by oxidation of fatty acid that can explain the observed differences.