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  Whole genome amplification : methods and protocols

  edited by Thomas Kroneis.

  Contents:
  Principles of whole-genome amplification / Zbigniew Tadeusz Czyz, Stefan Kirsch, and Bernhard Polzer
  Bias in whole genome amplification : causes and considerations / Jeffrey Sabina and John H. Leamon
  Single-cell lab or how to perform single-cell molecular analysis / Roland Kirchner and Marianna Alunni-Fabbroni
  Sample preparation methods following cellsearch approach compatible of single-cell whole-genome amplification : an overview / Joost F. Swennenhuis and Leon Terstappen
  Deterministic whole-genome amplification of single cells / Zbigniew Tadeusz Czyż and Christoph A. Klein
  Construction of a DNA library on microbeads using whole genome amplification / Takaaki Kojima, Bo Zhu, and Hideo Nakano
  Heat-induced fragmentation and adapter-assisted whole genome amplification using GenomePlex® single-cell whole genome amplification kit (WGA4) / Amin El-Heliebi, Shukun Chen , and Thomas Kroneis
  Whole genome amplification by isothermal multiple strand displacement using Phi29 DNA polymerase / Thomas Kroneis and Amin El-Heliebi
  Using multiplex PCR for assessing the quality of whole genome amplified DNA / Amin El-Heliebi, Shukun Chen, and Thomas Kroneis
  Quality control of isothermal amplified DNA based on short tandem repeat analysis / Thomas Kroneis and Amin El-Heliebi
  Laser microdissection of FFPE tissue areas and subsequent whole genome amplification by Ampli1 / Zbigniew Tadeusz Czyz, Nikolas H. Stoecklein, and Bernhard Polzer
  Whole genome amplification from blood spot samples / Karina Meden Sørensen
  Analysis of whole mitogenomes from ancient samples / Gloria Gonzales Fortes and Johanna L. A. Paijmans
  Copy number variation analysis by array analysis of single cells following whole genome amplification / Eftychia Dimitriadou, Masoud Zamani Esteki, and Joris Robert Vermeesch
  Whole genome amplification in genomic analysis of single circulating tumor cells / Christin Gasch, Klaus Pantel, and Sabine Riethdorf
  Whole genome amplification of labeled viable single cells suited for array-comparative genomic hybridization / Thomas Kroneis and Amin El-Heliebi
  Low-volume on-chip single-cell whole genome amplification for multiple subsequent analyses / Thomas Kroneis, Shukun Chen, and Amin El-Heliebi
  Detection and characterization of circulating tumor cells by the cellsearch approach / Frank Coumans and Leon Terstappen.

  Digital Access Springer 2015
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• Article

  Induction of multiple cytochrome P-450 species in housefly microsomes--SDS-gel electrophoresis studies.

  Stanton RH, Plapp FW, White RA, Agosin M.

  Comp Biochem Physiol B. 1978;61(2):297-305.

  1. Microsomal fractions isolated from various housefly strains have been characterized with respect to multiple forms of cytochrome P-450 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. Susceptible NAIDM houseflies were pretreated with known inducers of cytochrome P-450, and their microsomal electrophoretic profiles were compared to control NAIDM microsomes, using as standards partially purified cytochrome P-450s from noninduced NAIDM houseflies. 3. Tentatively, at least five different species of cytochrome P-450 may exist in the NAIDM housefly strain. 4. A comparison of the microsomal electrophoretic profile of different housefly strains also indicates the presence of at least two additional cytochrome P-450 species. 5. Induction with alpha-pinene and phenobarbital was expressed by a shift of the maximum absorbance at 452 nm in the CO-difference spectrum to lower wavelengths in the NAIDM strain; whereas, beta-naphthoflavone, although increasing the amount of cytochrome P-450, did not change the wavelength of maximum absorbance. 6. Cytochromes of the P-452 type appear to predominate in the susceptible NAIDM strain, while cytochromes of the P-450 and P-448 types predominate in resistant strains.

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  PubMed