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- Bookedited by Mar Orzáez, Mónica Sancho Medina, Enrique Pérez-Payá.Contents:
Immunoprecipitation of Cdy-Cyclin complexes for determination of kinase activity / Edurne Gallastegui and Oriol Bachs
Expression and purification of recombinant cyclins and CDKs for activity evaluation / Edurne Gallastegui and Oriol Bachs
Expression and purification of recombinant CDKs : CDK7, CDK8, and CDK9 / Reena Pinbero and Krassi,ir Yankulov
Preparation of CDK/Cyclin inhibitor complexes for structural determination / Asterios I. Grigoroudis and George Kontopidis
Fragment-based De Novo design of cyclin-dependent kinase 2 inhibitors / R Sunil Kumar Tripathi, Poonam Singh, and Sanjeev Kumar Singh
Protein-protein interaction for the De Novo design of cyclin-dependent kinase peptide inhibitors / Karthiga Arumugasamy [and three others]
Identification of cylin A bingers with a fluorescent peptide sensor / Elena Pazos, José L. Mascareñas, and Eugenio Vazquez
Cell synchronization techniques to study the action of CDK inhibitors / Beatriz Pérez-Benavente and Rosa Farrás
Analysis of CDK inhibitor action on mitochondria-mediated apoptosis / Anna Gortat
Evaluating the effects of CDK inhibitors is ischemia-reperfusion injury models / Tatiana Guevara
Assessing cell cycle independent function of the CDK inhibitor p21[superscript]CDKNIA in DNA repair / Ilaria Dutto, Micol Tillhon, and Ennio Properi
Drug delivery strategies of chemical CDK inhibitors / Daniel Alvira and Laura Mondragon
Animal models for studying the in vitro functions of cell cycle CDKs / Sanjiv Risal, Deepak Adhikari, and Kui Liu
Evaluating chemical CDK inhibitors as cell death inducers / Hiroshi Hirai and Yoko Nakatsuru.Digital Access Springer 2015 - ArticleRothstein M, Coppens M.Comp Biochem Physiol B. 1978;61(1):99-104.1. Soy-peptone has been fractionated to yield a series of increasingly purified components which sharply increase the populations of Caenorhabditis briggsae and Caenorhabditis elegans when added to the basal medium. The nutritionally active material appears to be a small polypeptide. 2. C. briggsae and C. elegans routinely reach populations of 150,000/ml or greater in 9 days in still culture, starting from an inoculum of only 500 organisms per ml. C. elegans is particularly sensitive to the depth of the medium. However, large populations can be achieved in deep cultures if continuous shaking is carried out. 3. Panagrellus silusiae shows improved populations if the basal medium is supplemented with the nutritional factor from soy-peptone. However, 0.5% acetic acid or 1% ethanol added to the medium serves equally well. There is no additive effect of ethanol and the factor.