Bookedited by Rainer Cramer, Reiner Westermeier.
Contents:
Part I Fundamentals
DIGE : past and future / Jonathan S. Minden
Basics of 2D DIGE / Phil Beckett
Multifluorescence 2D gel imaging and image analysis / Ingo Vormbrock, Sonja Hartwig, and Stefan Lehr
Assessing signal-to-noise in quantitative proteomics : multivariate statistical analysis in DIGE experiments / David B. Friedman
Analysis of proteins using DIGE and MALDI mass spectrometry / Witold M. Winnik [and others]
Synthesis and validation of cyanine-based dyes for DIGE / Michael E. Jung [and others]
Part II Methods
2D DIGE saturation labeling for minute sample amounts / Georg J. Arnold and Thomas Frohlich
Proteomic analysis of redox-dependent changes using cysteine-labeling 2D DIGE / Hong-Lin Chan, John Sinclair, and John F. Timms
Analysis of protein posttranslational modifications using DIGE-based proteomics / Robert M. DeKroon [and others]
Comparative analyses of protein complexes by blue native DIGE / Katrin Peters and Hans-Peter Braun
2D DIGE analysis of protein extracts from muscle tissue / Cecilia Gelfi and Sara De Palma
Combination of highly efficient hexapeptide ligand library-based sample preparation with 2D DIGE for the analysis of the hidden human serum/plasma proteome / Sonja Hartwig and Stefan Lehr
2D DIGE analysis of serum after fractionation by proteominertm beads / Cynthia Liang, Gek San Tan, and Maxey C.M. Chung
Study design in DIGE-based biomarker discovery / Alexandra Graf and Rudolf Oehler
Comparative 2D DIGE analysis of the depleted serum proteome for biomarker discovery / Megan Penno, Matthias Ernst, and Peter Hoffmann
Part III Applications in clinical proteomics
Differential gel-based proteomic approach for cancer biomarker discovery using human plasma / Keun Na [and others]
2D DIGE for the analysis of RAMOS cells subproteomes / Marisol Fernandez and Juan Pablo Albar
Application of saturation labeling in lung cancer proteomics / Gereon Poschmann [and others]
Proteomic profiling of the epithelial-mesenchymal transition using 2D DIGE / Rommel A. Mathias, Hong Ji, and Richard J. Simpson
Method for protein subfractionation of cardiovascular tissues before DIGE analysis / Athanasios Didangelos, Xiaoke Yin, and Manual Mayr
Application of DIGE and mass spectrometry in the study of type 2 diabetes mellitus mouse models / Celia Smith, Davinia Mills, and Rainer Cramer
Evaluating the efficacy of subcellular fractionation of blast cells using live cell labeling and 2D DIGE / Yin Ying Ho [and others]
Part IV Applications in animal, plant, and microbial proteomics
DIGE analysis of plant tissue proteomes using a phenolic protein extraction method / Christina Rode [and others]
Native DIGE of fluorescent plant protein complexes / Veronika Reisinger and Lutz Andreas Eichacker
Overview of 2D DIGE analysis of marine (environmental) bacteria / Ralf Rabus
Application of 2D DIGE in animal proteomics / Ingrid Miller.