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  • Book
    edited by Jean Peccoud.
    Contents:
    Part I. Assembly of oligonucleotides in synthons. Building block synthesis using the polymerase chain assembly method / Julie A. Marchand and Jean Peccoud. Oligonucleotide assembly in yeast to produce synthetic DNA fragments / Daniel G. Gibson. Topdown real-time gene synthesis / Mo Chao Huang [and others] De novo DNA synthesis using single-molecule PCR / Tuval Ben Yehezekel, Gregory Linshiz, and Ehud Shapiro
    Part II. Synthon assembly. SLIC : a method for sequence- and ligation-independent cloning / Mamie Z. Li and Stephen J. Elledge. Assembly of standardized DNA parts using bioBrick ends in E. coli / Olivia Ho-Shing [and others] Assembling DNA fragments by USER fusion / Narayana Annaluru [and others] Fusion PCR via novel overlap sequences / Kamonchai Cha-aim [and others] Using recombineering to generate point mutations : the oligonucleotide-based "Hit and Fix" method / Suhwan Chang, Stacey Stauffer, and Shyam K. Sharan. Using recombineering to generate point mutations : galK-based positive-negative selection method / Kajal Biswas, Stacey Stauffer, and Shyam K. Sharan. Assembling large DNA segments in yeast / Héloïse Muller [and others] Recursive construction of perfect DNA molecules and libraries from imperfect oligonucleotides / Gregory Linshiz, Tuval Ben Yehezkel, and Ehud Shapiro. Cloning whole bacterial genomes in yeast / Gwynedd A. Benders. Production of infectious poliovirus from synthetic viral genomes / Jeronimo Cello and Steffen Muller
    Part III. Software for gene synthesis. In silico design of functional DNA constructs / Alan Villalobos, Mark Welch, and Jeremy Minshull. Using DNAworks in designing oligonucleotides for PCR-based gene synthesis / David Hoover. De novo gene synthesis design using TmPrime software / Mo-Huang Li [and others] Design-a-gene with GeneDesign / Sarah M. Richardson [and others]
    Part IV. Education and security. Leading a successful iGEM team / Wayne Materi. The Build-a-Genome course / Eric M. Cooper [and others] DNA synthesis security / Ali Nouri and Christopher F. Chyba.
    Digital Access Springer 2012
  • Article
    Cassidy JT, Sullivan DB, Petty RE.
    Arthritis Rheum. 1977 Mar;20(2 Suppl):224-7.
    In the present study we have reconfirmed an earlier observation of the association of iridocyclitis with limited joint involvement in children with JRA. Children who develop iridocyclitis are predominantly girls whose sera are positive for antinuclear antibodies. Iridocyclitis was found to be asymptomatic at onset. Only periodic slit lamp examinations will detect the majority of cases. Iridocyclitis began a median of 2 years after onset of arthritis. Although most episodes occurred within 7 years after onset of arthritis, the risk of this development may never be absent in these children, even after they enter adulthood. In the present study, 16% of the afflicted children experienced either unilateral or bilateral blindness.
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